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目的:观察iNOS在血管再狭窄大鼠的表达变化及黄芪、当归的作用。方法:建立内皮剥脱后血管再狭窄动物模型,随机分为对照组、模型组、黄芪治疗组、当归治疗组、黄芪当归联合治疗组;治疗组在造模后给药,连续给药21 d,应用组织形态学、免疫组化的方法分别检测观察术后3,7,14,21 d iNOS在增生内膜中表达变化,并观察中药黄芪、当归注射液单独及联合应用对iNOS表达的影响。结果:正常主动脉内膜和中膜均检测到极少量iNOS表达;术后3 d大鼠主动脉壁内膜iNOS表达增强;7～21 d随着损伤时间延长及内膜的逐渐增厚,增生内膜iNOS阳性颗粒的数量增多,颜色变深,21 d达峰值;与模型组比较,黄芪、当归治疗21 d后,iNOS棕色颗粒明显减少,颜色变浅,以联合应用组作用更为明显。结论:iNOS表达参与内皮损伤后血管再狭窄过程,黄芪、当归可抑制血管内膜损伤后引起的血管内膜增生,其机制可能与抑制iNOS表达有关。
Objective: To observe the expression of iNOS in rats with vascular restenosis and the role of Astragalus and Angelica sinensis. Methods: Animal models of vascular restenosis after endothelial exfoliation were established and randomly divided into control group, model group, Astragalus membranaceus group, Angelica sinensis group and Astragalus angelica group. After treatment, the rats in treatment group were given continuous administration for 21 days, The changes of iNOS expression in proliferative endometrium were observed at 3, 7, 14 and 21 days after operation by histomorphology and immunohistochemistry. The effect of astragalus and angelica injection alone and in combination on iNOS expression was observed. Results: A very small amount of iNOS was detected in the intima and media of the normal aorta. The expression of iNOS in the aortic intima increased 3 days after operation. With the prolongation of injury time and the gradual thickening of the intima, The number of iNOS-positive particles in proliferating endometrium increased, and became darker on the 21st day. Compared with the model group, the iNOS brown particles were significantly reduced and the color was lighter after 21 days of treatment with Astragalus and Angelica, which was more obvious with the combined application group . CONCLUSION: iNOS expression is involved in the process of vascular restenosis after endothelial injury. Astragalus and angelica can inhibit the intimal hyperplasia induced by intimal injury, which may be related to the inhibition of iNOS expression.