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目的探讨18F-氟代脱氧葡萄糖(18F-FDG)和3-脱氧-3-18F-氟代胸腺嘧啶核苷(18F-FLT)的正电子发射断层显像(PET)对肿瘤放化疗的实验疗效。方法选用细胞株为LA-795的荷瘤小鼠96只,随机分为两组,放疗组48只和化疗组48只。放疗组荷瘤小鼠单次剂量为20 Gy,随机分为三组,16只未进行放疗为A组,16只放疗1 d后为B组,16只放疗2 d后为C组。化疗组荷瘤小鼠给予0.1 mg/mL顺铂腹腔注射,随机分为三组,16只未进行化疗为D组,16只化疗1 d后为E组,16只化疗2 d后为F组。将每组荷瘤小鼠平均分为两组,每组8只,分别给予18F-FDG和18F-FLT注射,1 h后处死,检测其注入剂量摄取率和增殖细胞抗原(PCNA)。结果依对照、放化疗1 d后、放化疗2 d后顺序,18F-FDG与18F-FLT的注入剂量摄取率和增殖细胞抗原均呈现出了下降趋势。相互比较时,18F-FDG的注入剂量摄取率下降差异不显著(P>0.05),18F-FLT的注入剂量摄取率、18F-FDG与18F-FLT的增殖细胞抗原下降差异显著(P<0.05)。18F-FDG注入剂量摄取率与增殖细胞抗原无相关性,18F-FLT注入剂量摄取率与增殖细胞抗原有相关性。结论18F-FDG和18F-FLT PET均可用于肿瘤放化疗疗效评价。18F-FLT对肿瘤放化疗疗效评价的价值要高于18F-FDG。
Objective To investigate the experimental efficacy of positron emission tomography (PET) with 18F-fluorodeoxyglucose (18F-FDG) and 3-deoxy-3-18F-fluorothymidine (18F-FLT) . Methods Ninety-six tumor-bearing mice with cell strain LA-795 were randomly divided into two groups, 48 in radiotherapy group and 48 in chemotherapy group. The single dose of tumor-bearing mice in the radiotherapy group was 20 Gy, which were randomly divided into three groups. Sixteen patients were treated with radiotherapy without radiotherapy. Group B was treated with 16 radiotherapy for 1 day and group C was treated with radiotherapy for 2 days. The tumor-bearing mice in the chemotherapy group were intraperitoneally injected with 0.1 mg / mL cisplatin, and were randomly divided into three groups. Sixteen rats were treated with chemotherapy without chemotherapy. Group D was given after 16 days of chemotherapy and group E was given after 16 days of chemotherapy. . Each group of tumor-bearing mice were equally divided into two groups, with 8 mice in each group. The mice were injected with 18F-FDG and 18F-FLT respectively. After 1 h, the mice were sacrificed and the dose-uptake rate and proliferating cell antigen (PCNA) were measured. Results According to the control, the dose-uptake rate of 18F-FDG and 18F-FLT and the proliferating cell antigen showed a decreasing trend after 1 day of chemoradiotherapy and 1 day after radiotherapy and chemotherapy. The difference of 18F-FDG dose-uptake rate was not significant (P> 0.05). The 18F-FLT injection dose rate, 18F-FDG and 18F-FLT decreased significantly . The uptake dose of 18F-FDG had no correlation with proliferating cell antigen, and the uptake dose of 18F-FLT had correlation with proliferating cell antigen. Conclusion Both 18F-FDG and 18F-FLT PET can be used to evaluate the efficacy of radiotherapy and chemotherapy in cancer. 18F-FLT on the efficacy of chemotherapy and radiotherapy to evaluate the value of higher than 18F-FDG.