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[目的]建立唑菌酯原药在0.5%羧甲基纤维素钠溶液(0.5%CMC)中的高效液相色谱(HPLC)分析方法,测定唑菌酯原药在0.5%CMC中的均一性。[方法]以乙腈-水(体积比70∶30)为流动相,流速为1.0 mL/min,使用Waters Symmetry C18色谱柱,紫外检测,波长254 nm,柱温35℃。[结果]低中高质量浓度样品平均回收率分别为98.0%～99.5%、97.7%～101%和94.9%～98.5%。批内精密度分别为0.1～1.6、0.4～4.1和1.5～2.0。批间精密度分别为0.8、1.7、1.9。线性相关系数大于0.999。配制的低高给药药剂的准确度分别为94.9%～96.4%和101%～103%。[结论]该方法具有简单、可靠、分离效果好、精密度和准确度高、线性关系好的特点,且给药药剂配制准确。
[Objective] To establish a HPLC method for the determination of pyraclostrobin in 0.5% sodium carboxymethylcellulose solution (0.5% CMC) to determine the homogeneity of pyraclostrobin in 0.5% CMC . [Method] The mobile phase was acetonitrile - water (volume ratio 70:30) and the flow rate was 1.0 mL / min. The detection wavelength was 254 nm with a Waters Symmetry C18 column and the column temperature was 35 ℃. [Result] The average recoveries of low, medium and high concentration samples were 98.0% -99.5%, 97.7% -101% and 94.9% -98.5%, respectively. Intra-batch precision were 0.1 ~ 1.6,0.4 ~ 4.1 and 1.5 ~ 2.0. Inter-batch precision were 0.8,1.7,1.9. The linear correlation coefficient is greater than 0.999. The accuracy of the formulated low hypoglycemic agents were 94.9% ~ 96.4% and 101% ~ 103%, respectively. [Conclusion] The method is simple, reliable, good separation effect, high precision and accuracy, good linear relationship, and the preparation of the drug is accurate.