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为揭示油菜BnGPAT9基因的功能及其在非生物胁迫中的表达变化,克隆甘蓝型油菜BnGPAT9基因(GenBank:KX344511.1)并分析表达模式。发现该基因全长1 131bp,编码376个氨基酸残基。序列比对及系统进化树分析表明BnGPAT9与其它植物的GPAT9有很大的相似性,在生物进化过程中具有很高的保守性。实时荧光定量PCR分析表明,BnGPAT9基因在甘蓝型油菜中的表达具有组织特异性,在开花后1~4周幼嫩的种子中表达较强,而在茎、花、叶、根和角果皮中表达较弱;5~6片真叶期盆栽苗的非生物胁迫表达分析显示,短期干旱和高温能够诱导和促进叶片BnGPAT9基因的表达;低温、6-BA、ABA和盐胁迫抑制其表达;水渍对BnGPAT9基因的表达影响呈“Z”字形,12h出现最低值,但24h后又开始回升。
In order to reveal the function of BnGPAT9 and its expression in abiotic stress, we cloned the BnGPAT9 gene (GenBank: KX344511.1) and analyzed the expression pattern. The gene was found to be 1 131 bp in length, encoding 376 amino acid residues. Sequence alignment and phylogenetic tree analysis showed that BnGPAT9 shared a great similarity with GPAT9 of other plants and was highly conserved in biological evolution. Real-time quantitative PCR analysis showed that the expression of BnGPAT9 gene in Brassica napus was tissue-specific and expressed strongly in the young seedlings 1 to 4 weeks after anthesis, while in the stems, flowers, leaves, roots and pericarp The expression of BnGPAT9 gene was induced and promoted by short-term drought and high temperature. The expression of BnGPAT9 gene was inhibited by low temperature, 6-BA, ABA and salt stress. The water Staining on the expression of BnGPAT9 gene was “Z ” glyph, 12h appeared the lowest value, but then began to rise after 24h.