论文部分内容阅读
目的 研究双色间期荧光原位杂交 (I-FISH)技术监测CML治疗过程中肿瘤细胞负荷的灵敏度及特异性。方法 应用I-FISH、常规细胞遗传学 (CCG)G显带技术和筑巢式逆转录聚合酶链式反应 (RT -PCR)技术对 2 0例治疗中CML患者骨髓中的肿瘤负荷进行检测。结果 对照组骨髓细胞假阳性率为 0 6 %～2 0 % ,分界值为 2 4 5 % ;2 0例患者经IFN -α治疗或骨髓移植后 9/18例(5 0 % )CCG检测到Ph(+)细胞 ,阳性细胞率 16 7%～ 10 0 % ;结合正常分界值 ,16 /2 0例 (80 % )经I-FISH检测到BCR -ABL(+)肿瘤细胞 ,阳性细胞率 2 8%～ 99 6 %。 16例患者行RT -PCR检查 ,13/16例 (81 3% )融合基因转录本阳性。结论 I -FISH技术可高度灵敏、特异地监测CML患者治疗过程中肿瘤细胞负荷的变化 ,与CCG及RT -PCR相比 ,检测所得结论更加科学、合理和具有说服力。
Objective To investigate the sensitivity and specificity of dual-color fluorescence in situ hybridization (I-FISH) to monitor the load of tumor cells during CML treatment. Methods The tumor burden in bone marrow of 20 CML patients undergoing therapy was detected by I-FISH, conventional cytogenetics (CCG) G banding and nested reverse transcriptase polymerase chain reaction (RT-PCR). Results The false positive rate of bone marrow cells was 0 6% ~ 20% in the control group, with a cut-off value of 24.5%. Twenty (20%) patients were detected by CCG after IFN-α treatment or bone marrow transplantation The positive rate of BCR-ABL (+) cells was 16.7% -10.0% for Ph (+) cells. The BCR-ABL (+) tumor cells were detected by I- 8% ~ 99 6%. Twenty-six patients underwent RT-PCR, and 13 of 16 (81.3%) fusion gene transcripts were positive. Conclusion The I-FISH technique can monitor the changes of tumor cell load in CML patients with high sensitivity and specificity. Compared with CCG and RT-PCR, the detection results are more scientific, reasonable and convincing.