论文部分内容阅读
①目的探讨青紫薯色素对β淀粉样肽诱导神经细胞毒性的保护作用及其机制。②方法建立β淀粉样肽诱导的PC-12神经细胞毒性病理模型,MTT法测定青紫薯色素对PC-12神经细胞增殖活性的影响,流式细胞仪检测PC-12神经细胞的凋亡率、线粒体膜电位、细胞内Ca2+浓度和Caspase-3的活性。③结果青紫薯色素可以剂量依赖性地提高PC-12细胞的增殖活性,降低β淀粉样肽诱导的PC-12细胞凋亡率,减轻线粒体膜的损伤,降低细胞内钙离子的浓度,抑制Caspase-3的活化。④结论青紫薯色素通过抑制Caspase-3的活化,减轻线粒体膜的损伤,降低细胞内Ca2+的浓度,起到减轻β淀粉样肽诱导的PC-12细胞损伤的作用。
Objective To investigate the protective effect of Qinghai potato pigment on neurotoxicity induced by β-amyloid peptide and its mechanism. 2Methods Pathological models of PC-12 neuronal cytotoxicity induced by β-amyloid peptide were established. MTT assay was used to determine the effect of Qingguangtai pigment on the proliferation activity of PC-12 nerve cells. Flow cytometry was used to detect the apoptosis rate of PC-12 neurons. Mitochondrial membrane potential, intracellular Ca2+ concentration, and Caspase-3 activity. 3 Results Qinghai potato pigment can increase the proliferation activity of PC-12 cells in a dose-dependent manner, reduce the rate of apoptosis of PC-12 cells induced by β-amyloid peptide, reduce the damage of mitochondrial membrane, reduce the concentration of intracellular calcium, and inhibit caspase -3 activation. 4 Conclusions Qinghai potato pigment can reduce the damage of mitochondrial membrane and decrease the concentration of intracellular Ca2+ by inhibiting the activation of Caspase-3, and it can reduce the damage of PC-12 cells induced by amyloid beta peptide.