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目的:探讨蛋白质组学技术用于动脉瘤性蛛网膜下腔出血(SAH)后脑血管痉挛(CVS)研究的可行性。方法:收集22例动脉瘤性SAH患者发病后第1天的脑脊液,按CVS程度将脑脊液标本分成3组:无痉挛组(对照组)、轻中度痉挛组、重度痉挛组。采用双向凝胶电泳分离各组混合脑脊液总蛋白,然后比较无痉挛组与轻中度痉挛组及重度痉挛组之间的双向凝胶电泳图谱。筛选出差异表达的蛋白质点,对差异点用4800 MALDI-TOF/TOF质谱仪进行鉴定。检索NCBInr数据库,确定差异表达的蛋白质。结果:无痉挛组与轻中度痉挛组及重度痉挛组差异表达2倍以上的蛋白质斑点有68个,成功鉴定并命名不同的差异表达蛋白23个。结论:运用蛋白质组学技术成功找到与CVS可能相关蛋白,为动脉瘤性SAH后CVS的发病机制的研究提供了新方法。
Objective: To investigate the feasibility of using proteomics in cerebral vasospasm (CVS) after aneurysmal subarachnoid hemorrhage (SAH). Methods: Cerebrospinal fluid (CSF) of 22 patients with aneurysmal SAH was collected on the first day after onset. The cerebrospinal fluid samples were divided into three groups according to the degree of CVS: no spasticity group (control group), mild to moderate spasticity group and severe spasticity group. Two-dimensional gel electrophoresis (SDS-PAGE) was used to separate the total CSF protein in each group. Two-dimensional gel electrophoresis (PLGE) was then performed between the spasm-free group and the mild to moderate spastic group and the severe spastic group. The differentially expressed protein spots were screened, and the differences were identified using a 4800 MALDI-TOF / TOF mass spectrometer. The NCBInr database was retrieved to identify differentially expressed proteins. RESULTS: Sixty-eight protein spots with more than 2-fold differential expression in spasm-free group and mild to moderate spasticity group and severe spasticity group were successfully identified and named 23 different differentially expressed proteins. CONCLUSIONS: The successful finding of proteins associated with CVS using proteomics provides a new method for studying the pathogenesis of CVS after aneurysmal SAH.