早先在膀胱癌,结肠癌中检测尿、大便中癌基因突变已获证实。为探索能在临床前期自痰中极少量的癌细胞中检出原癌基因突变的方法,本文选择15例过去未明确诊断,痰细胞学阴性,后来发展为肺腺癌、大细胞癌并手术的患者,重新检测贮藏痰标本中癌基因突变。组织学标本检测:将标本中DNA浸出,PCR扩增K-ras及P_(53)基因的突变。痰液标本的处理:将保存的痰 Early detection of oncogene mutations in bladder cancer and colon cancer has been confirmed. In order to explore methods for the detection of proto-oncogene mutations in cancer cells that can be detected in a small amount of preclinical autopsy, 15 cases were not diagnosed in the past and sputum cytology was negative. Later, lung adenocarcinoma, large cell carcinoma and surgery were developed. The patients were re-tested for oncogene mutations in stored sputum specimens. Histological specimen detection: DNA was leached from the specimen and PCR was used to amplify mutations in the K-ras and P_53 genes. Treatment of Sputum Samples: Saved Sputum