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目的:探讨急性心肌梗死(AMI)患者外周血Th22和Th17细胞,及其细胞因子白细胞介素(IL)-22和IL-17的变化及临床意义。方法:冠心病患者(CHD)34例,其中AMI患者19例(AMI组)、稳定型心绞痛(SAP)患者15例(SAP组),另取15例冠状动脉造影正常者作为健康对照组(HC组);采用流式细胞技术检测各组外周血Th22和Th17细胞在CD4+T细胞中的比例;ELISA法检测血浆IL-22和IL-17浓度。结果:AMI组外周血Th22细胞(2.43±1.77)%、Th17细胞(2.80±1.15)%,均显著高于SAP组(0.92±0.26)%,(1.59±0.49)%及HC组(0.84±0.19)%、(1.35±0.32)%(均P<0.05),SAP组与HC组差异无统计学意义。AMI组血浆IL-22(33.74±7.25)pg/ml、IL-17(24.95±8.86)pg/ml浓度均显著高于SAP组(26.95±3.19)pg/ml,(18.86±5.65)pg/ml及HC组(24.17±2.55)pg/ml、(14.63±2.57)pg/ml(均P<0.05),SAP与HC组差异无统计学意义。AMI组患者的Th22细胞比例与Th17细胞比例(r=0.525,P<0.05)、IL-22浓度(r=0.771,P<0.01)均呈显著正相关,AMI组的Th17细胞比例与IL-17浓度也呈显著正相关(r=0.627,P<0.01)。结论:外周血Th22、Th17细胞及其相关细胞因子在AMI的发生中起重要作用。
Objective: To investigate the changes of Th22 and Th17 cells, interleukin (IL) -22 and IL-17 in peripheral blood of patients with acute myocardial infarction (AMI) and their clinical significance. Methods: Thirty-four patients with coronary heart disease (CHD), including AMI (AMI), 15 patients with stable angina (SAP) and 15 healthy controls Group). The proportion of Th22 and Th17 cells in CD4 + T cells in each group was detected by flow cytometry. The concentrations of IL-22 and IL-17 in plasma were detected by ELISA. Results: The levels of Th22 cells (2.43 ± 1.77)% and Th17 cells (2.80 ± 1.15)% in AMI group were significantly higher than those in SAP group (0.92 ± 0.26)%, (1.59 ± 0.49)% and HC group ), (1.35 ± 0.32)% (all P <0.05). There was no significant difference between SAP group and HC group. The concentrations of plasma IL-22 (33.74 ± 7.25) pg / ml and IL-17 (24.95 ± 8.86) pg / ml in AMI group were significantly higher than those in SAP group (26.95 ± 3.19 pg / ml, 18.86 ± 5.65 pg / ml And HC group (24.17 ± 2.55) pg / ml, (14.63 ± 2.57) pg / ml respectively (all P <0.05). There was no significant difference between SAP group and HC group. The proportion of Th22 cells in AMI patients was positively correlated with the proportion of Th17 cells (r = 0.525, P <0.05) and IL-22 concentration (r = 0.771, P <0.01) There was also a significant positive correlation (r = 0.627, P <0.01). Conclusion: Th22 and Th17 cells in peripheral blood and related cytokines play an important role in the pathogenesis of AMI.