论文部分内容阅读
目的研究尾吊、噪声及两因素复合对小鼠免疫功能的影响及柘木多糖的保护作用。方法利用MTS比色法测定淋巴细胞增殖,ELISA法测定细胞因子,观察7 d尾吊、2 h噪声及两因素叠加处理对小鼠脾T淋巴细胞增殖、细胞因子产生以及腹腔巨噬细胞细胞因子产生的影响。结果 2 h噪声能提高Th1细胞免疫功能,但抑制巨噬细胞功能,7 d尾吊对Th1、Th2细胞免疫功能具有抑制作用,但能刺激巨噬细胞功能;柘木多糖对尾吊、噪声叠加应激引起的T淋巴细胞增殖下降、IL-2分泌减少、巨噬细胞TNF-α产生下降有一定的提升作用。结论急慢性应激因素对免疫细胞的影响具有细胞特异性;柘木多糖对尾吊和噪声这两种急慢性应激引起的免疫功能下降有一定的防护作用。
Objective To study the effects of tail-hang, noise and two-factor combination on immune function and the protective effect of Alnus cremastogyne polysaccharides. Methods Lymphocyte proliferation was measured by MTS colorimetric assay. The cytokines were measured by ELISA. The effects of 7 d tail suspension, 2 h noise and two factors superposition on the proliferation of splenic T lymphocytes, cytokine production, and cytokines in peritoneal macrophages The impact of. Results The noise of 2 h increased the immune function of Th1 cells, but inhibited the function of macrophages. The 7 d tail suspension inhibited the immune function of Th1 and Th2 cells but stimulated the function of macrophages. Induced T lymphocyte proliferation decreased, IL-2 secretion decreased macrophage TNF-α production decreased to some extent. Conclusion The effects of acute and chronic stress factors on the immune cells are cell specific. The polysaccharides of Alnus japonicus have a protective effect on the immune function decline caused by both acute and chronic stress, tail and hang.