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目的:观察芩荑合剂对哮喘大鼠肺组织模型信号转导子和转录激活子(STAT6)蛋白含量及其mRNA表达影响,探讨芩荑合剂对支气管哮喘的作用机理。方法:用卵清蛋白建立大鼠哮喘模型,将48只SD大鼠随机分为正常对照组、哮喘模型组、地塞米松组、芩荑合剂低剂量组、芩荑合剂中剂量组、芩荑合剂高剂量组,其中正常对照组、哮喘模型组给予生理盐水灌胃,芩荑合剂高、中、低剂量分别按生药量5.4、2.70、1.35 g/(kg·d)灌胃,地塞米松按1.4 mg/(kg·d)灌胃,采集肺组织标本,分别采用实时荧光定量PCR(Realtime PCR)法和免疫组化法检测哮喘大鼠模型STAT6m RNA及其蛋白表达。结果:中、高剂量组STAT6 mRNA及蛋白表达较哮喘组显著减弱,有显著差异(P<0.01),与地塞米松组比较无统计学意义。结论:芩荑合剂通过降低哮喘大鼠STAT6蛋白含量以及减弱STAT6 mRNA在气道上皮细胞的表达,来干预气道炎症,从而达到治疗支气管哮喘的目的。
Objective: To observe the effect of Qinhuang mixture on the expression of signal transducer and activator of transcription 6 (STAT6) and its mRNA in the lung tissue of asthmatic rats, and explore the mechanism of Qinhuang mixture on bronchial asthma. Methods: A rat model of asthma was established with ovalbumin. 48 SD rats were randomly divided into normal control group, asthma model group, dexamethasone group, Qinlian Mixture low dose group, Qinlian Mixture medium dose group, High dose group, in which normal control group, asthma model group were given physiological saline, Qinhuang mixture of high, medium and low doses of raw drugs were 5.4,2.70,1.35 g / (kg · d) gavage, dexamethasone The lung tissue samples were collected by gavage at a dose of 1.4 mg / (kg · d), and STAT6mRNA and its protein expression in the asthmatic rat model were detected by Realtime PCR and immunohistochemistry respectively. Results: STAT6 mRNA and protein expression in middle and high dose groups were significantly lower than those in asthma group (P <0.01), but no significant difference compared with dexamethasone group. Conclusion: Qinhuang Mixture can reduce the level of STAT6 protein in asthmatic rats and attenuate the expression of STAT6 mRNA in airway epithelial cells to interfere with airway inflammation and achieve the purpose of treating bronchial asthma.