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目的 探讨增生性瘢痕 (HS)和瘢痕疙瘩 (Ks) ,角朊细胞 (KC)表达 ICAM- 1和 HL A- DR) ,及≤ 1m o的 HS(HS1) ,2～ 6 m o的 HS(HS2 )和 7～ 2 4mo的 HS(HS3)标本 ,分别作抗 ICAM- 1,HL A- DR,CD3,CD4和 CD8的 Mc Ab的SABC方法免疫组化染色和观察 .结果 各组 KC表达 I-CAM- 1和 HL A- DR抗原的阳性检出率 (% )、CD3+ 细胞密度(cells/ mm2 )及 CD4+ / CD8+比值 (x±s) ,并作统计学处理 .各组标本的 KC表达 ICAM- 1和 HL A- DR抗原的阳性检出率依次为 :Ks- I>Ks- P>Ks- A,HS1>HS2 >HS3(t,P<0 .0 5～0 .0 0 1) .各组 KC表达 ICAM- 1和 HL A- DR的阳性率与组织内浸润的 CD3+细胞密度和 CD4+ / CD8+比值呈明显正相关 (P<0 .0 5～ 0 .0 1,r=0 .2 49～ 0 .32 4) .结论 KS和 HS的 KC通过表达 ICMA- 1和 HL A- DR抗原 ,诱导 T细胞浸润 ,上调局部免疫炎性反应 ,可能是病理性瘢痕形成的重要因素
Objective To investigate the expression of ICAM-1 and HL A-DR in hypertrophic scars (HS) and keloids (Ks), keratinocytes (HSCs) ) And 7 ~ 24mo HS (HS3) specimens were used to detect the expression of ICAM-1, HL-DR, CD3, CD4 and CD8 Mc Ab SABC immunohistochemical staining and observation.Results KC in each group expression of I- The positive detection rate (%), CD3 + cell density (mm2) and CD4 + / CD8 + ratio (x ± s) of CAM-1 and HL A-DR antigen were calculated and statistically analyzed. The positive detection rates of ICAM-1 and HL A-DR antigen were as follows: Ks-I> Ks-P> Ks-A and HS1> HS2> HS3 (t, P <0.05-0.010) The positive rate of ICAM-1 and HL-DR expression in KC group was positively correlated with the infiltration of CD3 + cell density and CD4 + / CD8 + ratio in each group (P <0.05-0.01, r = 2 49 ~ 0 .32 4) .Conclusion KC of KS and HS can induce T cell infiltration and up-regulate local immunoinflammatory reaction by expressing ICMA-1 and HL A-DR antigen, which may be an important factor of pathological scar formation