论文部分内容阅读
甜樱桃品种‘拉宾斯’与‘雷尼’均为S1S4基因型。调查其自花结实率,‘拉宾斯’为31.3%,表现自交亲和;‘雷尼’为0,表现为自交不亲和。将‘拉宾斯’与‘雷尼’相互授粉,‘拉宾斯’为父本时表现亲和,反之不亲和,推断‘拉宾斯’的自交亲和性由花粉侧突变导致;调查花粉萌发率,‘拉宾斯’为58.08%,‘雷尼’为57.82%。鉴定‘拉宾斯’自交后代及其与‘雷尼’杂交后代的S-RNase的基因型,发现所有后代中均出现S4基因型,部分后代中出现S1基因型,证明‘拉宾斯’花粉育性是正常的,其亲和性可能是由S4基因座上花粉侧的基因突变导致。测序发现‘拉宾斯’花粉SFB4′在911 bp处有4个碱基缺失。分析‘拉宾斯’花粉SFB4′与花柱S4-RNase的连锁性,发现两者连锁率为100%。以‘拉宾斯’为父本的带有SFB4′的杂交后代的自花结实率为23.3%~61.4%,表现自交亲和,说明来源于‘拉宾斯’的SFB4′基因与自交亲和性有关。RT-PCR及测序显示‘雷尼’SFB4与‘拉宾斯’SFB4′均能正常转录,但4个碱基缺失导致其C端翻译提前终止,原核表达SFB4和SFB4′蛋白发现SFB4略大于SFB4′,推测是由于SFB4′的翻译提前终止导致的。以上结果表明:‘拉宾斯’的自交亲和性可能是由花粉侧SFB4′C端的氨基酸缺失导致,该缺失可能使其丧失了与S-RNase的识别能力。
The sweet cherry varieties ’Labins’ and ’Raney’ are S1S4 genotypes. To investigate the self-fertility rate, ’Labins’ was 31.3%, showing self-compatibility; ’Raney’ was 0, manifested as self-incompatibility. ’Rabins’ and ’Raney’ were pollinated with each other, while ’Labins’ showed affinity when they were their parents and conversely, they concluded that the self-compatibility of ’Labins’ was caused by pollen mutation. Pollen germination rates were investigated for ’Labins’ at 58.08% and ’Raney’ at 57.82%. The genotypes of S-RNase in ’Labin’ selfing offspring and their hybrids with ’Raney’ were identified, and the S4 genotype was found in all offspring. The S1 genotype was found in some offspring, indicating that ’Labins’ Pollen fertility is normal, and its affinity may be caused by a genetic mutation in the pollen side of the S4 locus. Sequencing revealed that ’Labs’ pollen SFB4’ had 4 base deletions at 911 bp. The linkage between ’Labin’ pollen SFB4 ’and style S4-RNase was analyzed and found that the linkage rate between the two was 100%. The selfing rate of hybrids with SFB4 ’derived from’ Labins ’was from 23.3% to 61.4%, showing self-compatibility, indicating that SFB4’ gene from ’Labins’ Affinity related. RT-PCR and sequencing showed that both Raney SFB4 and ’Rabins’ SFB4 ’could be normally transcribed, but the deletion of 4 bases led to the early termination of C-terminal translation. SFB4 and SFB4’ prokaryotic expression proves that SFB4 is slightly larger than SFB4 ’, Presumably due to early termination of SFB4’ translation. The above results indicate that the self-compatibility of ’Labins’ may be caused by the lack of amino acids at the pollen-side SFB 4’C, which may cause it to lose its ability to recognize S-RNase.