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人乳头瘤病毒（ＨＰＶ）感染与宫颈癌的发生关系密切。其晚期蛋白Ｌ１是主要结构蛋白，可以刺激机体产生中和抗体，对病毒攻击可以起到保护作用。本研究将ＨＰＶ１６型中国分离株的Ｌ１基因定向克隆到原核表达载体ｐＥＴ－２１ｃ质粒中，建立ＨＰＶ１６ Ｌ１在大肠杆菌中的高效表达系统ｐＥＴ２１ｃ－ＨＰＶ１６Ｌ１，该系统在ＩＰＴＧ的诱导下可表达 ６０ｋｕ的 Ｌ１蛋白，表达效率为 ２３％，此蛋白通过Ｗｅｓｔｅｒｎ－ｂｌｏｔ得到证实。该表达体系的成功建立可以为进一步研究ＨＰＶ１６ Ｌ１蛋白的分子和免疫学特性以及基因疫苗的研制提供实验依据。
Human papillomavirus (HPV) infection and the occurrence of cervical cancer are closely related. Its late protein L1 is the main structural protein that can stimulate the body to produce neutralizing antibodies, which can play a protective role against virus attack. In this study, the L1 gene of HPV16 type Chinese isolate was cloned into the prokaryotic expression vector pET-21c. The recombinant plasmid pET21c-HPV16L1 was successfully constructed in E.coli. The system can express 60ku L1 under the induction of IPTG Protein, the expression efficiency of 23%, this protein was confirmed by Western-blot. The successful establishment of this expression system can provide experimental evidence for further study on the molecular and immunological properties of HPV16 L1 protein and the development of gene vaccines.