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报道了用基因枪转化法将雪花莲凝集素基因 (gna)转移到优良籼型杂交稻恢复系蜀恢 5 2 7中 .PCR、PCR -Southernblotting和Southernblotting等分子检测证明 ,外源基因以多拷贝单位点方式整合到受体基因组中并稳定遗传到转基因第三代 (T2 ) .转基因第一代植株 (T0 )在株高和结实率上与相应的组培、种子实生苗植株相比 ,发生明显的不可遗传的变异 ,随着繁殖代数的增加 ,转基因植株恢复到与对照植株一致 .Westernblotting表明 ,目的基因在转基因植株中正确表达 .蛋白活性分析显示 ,外源基因在转基因植株中合成的蛋白具有凝血生物活性 ,含量占可溶性总蛋白的0 .1%左右 .本文还对转基因技术在杂交稻育种中的应用进行了讨论 .图 4表 2参 18
It was reported that the gna gene was transferred into the restorer line Shuhui 57 27 of the indica hybrid rice by gene gun transformation.PCR, PCR-Southern blotting and Southern blotting showed that the exogenous gene was expressed in multiple copies The haplotype integration into the recipient genome and stable inheritance to the third generation of transgenic (T2) transgenic first generation (T0) in plant height and seed setting rate with the corresponding tissue culture, seedling seedlings compared to occur Significantly non-heritable variation, with the increase of breeding algebra, the transgenic plants returned to the same as the control plants.Westernblotting showed that the target gene was correctly expressed in the transgenic plants.Analysis of protein activity showed that the foreign genes synthesized in the transgenic plants With coagulation biological activity, the content of total soluble protein accounted for about 0.1% .This paper also discussed the application of transgenic technology in hybrid rice breeding .Figure 4 Table 2 Reference 18