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目的探讨乙型肝炎(乙肝)患者乙肝病毒外膜大蛋白(BHV-LP)及与乙肝病毒DNA(HBV DNA)关联性。方法选取2014—2015年湘雅萍矿合作医院收治的288例乙肝患者为乙肝组,并选取同期的健康体检者50例为对照组,应用实时荧光定量聚合酶链式反应(PCR)技术和酶联免疫吸附试验(ELISA)检测HBV DNA及HBV-LP。结果乙肝组HBV DNA阳性188例(65.3%);HBV-LP阳性194例(67.4%)。对照组50例HBVLP与HBV DNA均为阴性。HBV-LP与HBV DNA呈正相关(r=0.175,P<0.05)。乙肝患者HBe Ag阳性127例,其中HBV DNA阳性112例,阳性率88.2%;HBV-LP阳性106例,阳性率为83.5%。HBV DNA与HBV-LP阳性率比较,差异无统计学意义(P>0.05)。结论 HBV-LP可作为一种病毒复制的血清学指标。
Objective To investigate the association between hepatitis B virus outer membrane protein (BHV-LP) and hepatitis B virus DNA (HBV DNA) in patients with hepatitis B (hepatitis B). Methods A total of 288 hepatitis B patients admitted to Xiangyaping Coal Mine Cooperation Hospital from 2014 to 2015 were selected as hepatitis B patients and 50 healthy subjects were selected as control group. Real-time fluorescence quantitative polymerase chain reaction (PCR) and enzyme Anti-HBV DNA and HBV-LP were detected by ELISA. Results Hepatitis B virus DNA positive 188 cases (65.3%); HBV-LP positive 194 cases (67.4%). The control group of 50 cases of HBVLP and HBV DNA were negative. HBV-LP was positively correlated with HBV DNA (r = 0.175, P <0.05). HBe Ag was positive in 127 hepatitis B patients, of which 112 were positive for HBV DNA, the positive rate was 88.2%. The positive rate of HBV-LP was 106 (83.5%). HBV DNA and HBV-LP positive rate, the difference was not statistically significant (P> 0.05). Conclusion HBV-LP can be used as a serological marker of viral replication.