白僵病是柞蚕病害之一,在通常情况下自然发病率很低,不是柞蚕主要病害。近年来,进行生物防治使用白僵菌的数量有所增加,这对减少化学农药的用量、保护天敌、防止环境和食品污染等起了积极作用。但同时是否会给柞蚕生产带来影响,是个值得注意的问题。为此我们进行了白僵菌对柞蚕幼蚕寄生力的测定试验。一、试验材料1.供试菌粉由金县三十里堡生物农药厂提供,每克菌粉含孢子100亿。2.供试柞蚕春季取盖县生防站柞蚕茧,置于5℃冰箱中贮存,试验前取出蚕茧放于室内,使其自然羽化、交尾、产卵,并孵出幼蚕。二、试验方法将初孵柞蚕幼蚕在虫笼内放养,饲以鲜苹果叶,两天后选健壮个体用于试验。把白僵菌粉加无菌水配制成孢子含量为0.4265亿/毫升 White stiff disease is one of the tussah diseases, the natural incidence is very low under normal circumstances, not the main disease tussah. In recent years, there has been an increase in the use of Beauveria bassiana for biological control, which has played a positive role in reducing the amount of chemical pesticides, protecting natural enemies and preventing environmental and food contamination. But at the same time whether it will affect the production of tussah, is a noteworthy issue. To this end, we conducted Beauveria bassiana on tussah silkworm parasitism determination test. First, the test material 1 for the test powder by the county gold thirty miles Fort Pesticide Factory, per gram of bacteria containing spore 10 billion. 2 for the test tussah taking cover County Health Station tussah cocoon spring, placed in a 5 ℃ refrigerator, remove the cocoon before the test indoors, so that it naturally eclosion, mating, spawning, and hatch baby silkworm. Second, the test method The newly hatched tussah silkworm baby silkworm stocking, feeding fresh apple leaves, two days after the election robust individual for testing. Beauveria bassiana powder plus sterile water spores formulated for the spore content of 0.4265 million / ml