【摘 要】
：
目的:探讨如何在外周血淋巴细胞培养染色体畸变分析中做好质量控制,以获得良好的涂片效果.方法:在已消毒好备用的超净台内,用碘伏消毒采血管的头部,轻轻混匀后接种,加入秋水
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目的:探讨如何在外周血淋巴细胞培养染色体畸变分析中做好质量控制,以获得良好的涂片效果.方法:在已消毒好备用的超净台内,用碘伏消毒采血管的头部,轻轻混匀后接种,加入秋水仙素工作液,经混匀、培养、收集细胞并离心后,弃上清液,加入低渗液混匀,给予预固定;重复固定后依细胞数量加入适量固定液,制片、染色、气干.于低倍镜下细致观察,计数200 个分裂相.结果:细胞生长良好,分裂指数高,能够满足分析要求,染色体分散均匀,着色良好,长短适中,两条姐妹染色体大致平行分离,着丝粒清晰.结论:做好实验室内质量控制能提高染色体畸变的检出率和阅片速度,及时准确地反映辐射损伤情况,对放射工作人员健康进行监护,为意外放射性损伤事故进行生物剂量估算,放射患者诊断与治疗提供依据.
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