研究棉花黄萎病,常需要进行病菌单孢子分离,由于轮枝菌孢子小而无色,极易与培养基的微小质粒混淆,应用文献中已报道的方法很难快速而准确地挑取棉花黄萎病菌单孢。笔者在实践中,试用琼胶平板孢子双芽管萌发法,效果准确可靠,操作简便。现简述如下: Studies on Verticillium wilt disease in cotton often require single spore isolation of pathogens. Because of the small and colorless spores of Verticillium sp., Which are easily confused with the tiny plasmids of the culture medium, the methods reported in the literature have made it difficult to pick cotton quickly and accurately Verticillium wilt single spore. In practice, the author try agar plate spore two germination method, the effect is accurate and reliable, easy to operate. Are summarized as follows: