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目的观察益气活血中药对糖尿病大鼠心肌组织羟脯氨酸含量的影响。方法予高脂高糖饲料喂养的雄性SD大鼠一次性腹腔注射50 mg/kg链脲佐菌素(STZ)建立糖尿病模型,4周后以益气活血方及其有效组分配方进行药物干预,同时设正常对照组及模型对照组。观察大鼠的一般情况,灌胃4周后处死大鼠,剪取左心室心肌组织,采用HE染色观察大鼠心肌组织形态学,酶联免疫吸附试验(ELISA)法检测大鼠心肌组织羟脯氨酸含量。结果与正常对照组相比,模型对照组大鼠一般情况较差,心肌病理损害严重,心肌羟脯氨酸含量明显增加,益气活血中药干预后各组大鼠以上情况得到不同程度改善。结论益气活血中药可能通过减少心肌组织羟脯氨酸含量发挥抗糖尿病大鼠心肌纤维化的作用。
Objective To observe the effect of Yiqi Huoxue Chinese medicine on hydroxyproline content in myocardium of diabetic rats. Methods Male Sprague-Dawley rats fed with high-fat and high-sucrose diet were given a single intraperitoneal injection of streptozotocin (STZ) at a dose of 50 mg / kg to establish a diabetic model. After 4 weeks, the drug was given to Yi-qi Huoxue decoction and its effective components , While the normal control group and model control group. The rats were observed. The rats were sacrificed after 4 weeks of gavage, the left ventricular myocardium was cut out and the morphology of myocardial tissue was observed by HE staining. The myocardial tissue of the rats was examined by enzyme-linked immunosorbent assay (ELISA) Amino acid content. Results Compared with the normal control group, the rats in the model control group were generally poor, the pathological changes of myocardium were serious, and the contents of hydroxyproline in the myocardium were significantly increased. The above conditions were improved to some extent after the intervention of Yiqi Huoxue Chinese medicine. Conclusion Yiqi Huoxue Chinese medicine can exert antimyocardial fibrosis in diabetic rats by decreasing the content of hydroxyproline in myocardium.