一、目的与材料方法 环剥在柑桔树上的应用,在我国已有悠久的历史。但常规环剥再生速度慢,环剥宽度难于掌握。近年来,我们对柑桔环剥后包以薄膜,剥口暴露的木质部能在2个月左右重新长出新的树皮,研究了树皮再生的解剖发生。 所用试材取自于本所12年生枳砧华盛顿脐橙。最初的环剥试验始于1988年,而大部分环剥则在1990年进行。4月20日,选长势较强,生长较一致的10株树,在一株的2～3个直径3～5cm的大枝上进行环剥。方法是用刀将枝环切两圈,深达木质部,两圈之间再竖切一刀,挑起树皮,将其剥离,剥口宽3cm。剥后包以无色聚乙烯薄膜保湿。一个月后解膜。制片观察的材料按下列时间取材:环剥前,环剥当天和剥后1、3、6、10、15、20天和1、2、3、4个月,及剥后1年(1989年剥)和2年(1988年剥)。每次取材固定时,从每株树上切取3～5块1.0cm×0.25～0.5cm小块,稍带木质部,置于FAA固定液中。固定后的材料用石蜡切片法切片,切片厚度8～12μm,铁矾—苏木精—番红染色。 二、结果 First, the purpose and method of material Girdling in the application of citrus trees, in our country has a long history. However, the slow rate of conventional girdling regeneration, girdling width is difficult to grasp. In recent years, we have re-created new barks about 2 months after exposure to thin strips of citrus girdling and exfoliated xylem, and studied the anatomical occurrence of bark regeneration. Specimens from the 12-year-old orange lotus root Washington Orange. The initial girdling test began in 1988, while most of the girdling took place in 1990. On April 20, ten trees with stronger growth and more consistent growth were girdled on 2 ~ 3 large branches of 3 ~ 5cm in diameter. The method is to use a knife to cut the branch ring twice, reaching the xylem, then cut between two circles and then cut a knife, provoke the bark, the peel, stripping wide 3cm. Peel after the pack with a colorless polyethylene film moisturizing. A month after the solution. The materials observed for the filming were taken on the following days: before girdling, on the same day of girdling and at 1, 3, 6, 10, 15, 20 days and 1, 2, 3, 4 months after peeling and 1 year after peeling Year peel) and 2 years (peel in 1988). Each time a sample is fixed, 3 to 5 pieces of 1.0 cm × 0.25 to 0.5 cm pieces are cut from each tree, slightly xylem, and placed in FAA fixative. The fixed material was sectioned by paraffin method and sliced ​​to a thickness of 8 ~ 12μm with alum-hematoxylin-safranin staining. Second, the result