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目的:应用生物信息学方法预测H1N1亚型流感病毒血凝素Th和B细胞相关抗原表位,并初步分析其抗原性,为研制H1N1亚型流感病毒的表位疫苗奠定基础。方法:依据近年流感病毒流行趋势,从GenBank下载具有代表性的H1N1亚型流感病毒HA蛋白氨基酸序列。进行生物信息学综合分析预测,获得Th和B细胞相关抗原表位,并比较其保守性和特异性。通过Balb/c小鼠H1N1亚型流感病毒阳性血清与表位肽的结合试验,初步鉴定候选表位抗原性。结果:综合多项预测及空间构像模拟结果,我们获得了三条候选Th和B细胞表位,分别为HA73～87、HA125～139、HA188～205。候选表位处于H1N1亚型流感HA1蛋白序列上相对保守的区域内,且与目前流行的H1N1亚型流感病毒HA相应区域具有较好的一致性。而不同候选表位在BALB/c小鼠H1N1亚型流感病毒阳性血清反应中显示了不同抗体结合能力,预示了其成为功能表位的可能。结论:所筛选的表位具有成为H1N1亚型流感病毒HATh和B细胞相关抗原表位的可能。此研究为深入揭示流感病毒感染与免疫机制,H1N1亚型流感功能表位认知及表位疫苗研究奠定了基础。
OBJECTIVE: To predict the antigenicity of hemagglutinin Th and B cell associated antigen of H1N1 influenza virus by bioinformatics method, and to lay a foundation for the development of the epitope vaccine of H1N1 subtype influenza virus. Methods: Based on the epidemic trend of influenza virus in recent years, the HA protein amino acid sequence of influenza virus subtype H1N1 was downloaded from GenBank. Bioinformatics comprehensive analysis of prediction, access to Th and B cell-related antigen epitopes, and to compare their conservation and specificity. Candidate epitope antigenicity was initially identified through the binding test of the positive serum of H1N1 subtype influenza virus of Balb / c mice with the epitope peptide. Results: Based on the results of multiple predictions and spatial image simulations, we obtained three candidate Th and B cell epitopes, HA73 ~ 87, HA125 ~ 139 and HA188 ~ 205, respectively. The candidate epitopes are in a relatively conservative region of the HA1 protein sequence of the H1N1 influenza A virus and have good consistency with the corresponding HA region of the currently circulating H1N1 influenza virus HA. Different candidate epitopes showed different antibody binding ability in the positive serological response of H1N1 subtype influenza virus of BALB / c mice, indicating that they may become functional epitopes. Conclusion: The selected epitopes have the potential to become HATh and B cell related antigen epitopes of H1N1 influenza virus. This study lays a foundation for further revealing influenza virus infection and immune mechanism, cognition of H1N1 subtype influenza functional epitope and epitope vaccine research.