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目的 运用基因芯片技术研究变应性鼻炎的基因表达谱。方法 对变应性鼻炎和非变应性鼻炎鼻黏膜组织进行总RNA抽提 ,纯化后的mRNA进行逆转录制备杂交探针 ,应用含有 12 80 0个人类全长互补DNA(complementaryDNA ,cDNA)的表达谱芯片对 8例变应性鼻炎与非变应性鼻炎鼻黏膜组织进行差异表达谱分析。结果 在变应性鼻炎的基因表达谱中差异表达基因共有 734条 ,上调的基因 4 30条 ,下调的基因 30 4条 ,共存性差异表达基因有 2 1条 ,其中上调基因 15条 ,下调基因 6条 ,在差异表达的基因中 ,其中有些是炎性反应、免疫应答、免疫调控、神经内分泌网络系统及信号传导的相关基因 ,这些基因可能与变应性鼻炎发病机制有关。结论 运用基因芯片技术研究变应性鼻炎相关基因 ,可能为鼻变态反应性疾病的病因和发病机理的研究提供新思路
Objective To study the gene expression profile of allergic rhinitis using gene chip technology. Methods Total RNA was extracted from nasal mucosa of allergic rhinitis and non-allergic rhinitis. The purified mRNA was reverse-transcribed to prepare hybridization probe. The hybridization probe containing 12 80 0 complementary DNA (cDNA) Differential expression profiling of nasal mucosa in 8 patients with allergic rhinitis and non-allergic rhinitis was performed with the expression profiling chip. Results There were 734 differentially expressed genes, 4 30 up-regulated genes and 30 4 down-regulated genes in the gene expression profiles of allergic rhinitis. There were 21 differentially expressed genes in which there were 15 up-regulated genes and down-regulated genes Six of the differentially expressed genes, some of which are inflammatory response, immune response, immune regulation, neuroendocrine network system and signal transduction related genes, these genes may be related to the pathogenesis of allergic rhinitis. Conclusion Using gene chip technology to study the related genes of allergic rhinitis may provide new ideas for the etiology and pathogenesis of nasal allergic diseases