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目的:探讨辛伐他汀在Ｈ２Ｏ２引致的动脉内皮细胞急性损伤中的作用。方法:取ＳＤ大白鼠的胸主动脉制成３~４ｍｍ的血管环。损伤模型组:用５ｍｍｏｌＨ２Ｏ２与血管环孵浴１５ｍｉｎ;辛伐他汀组:先用５０μｍｏｌ辛伐他汀与血管环孵浴２５ｍｉｎ,冲洗后,再用５ｍｍｏｌ Ｈ２Ｏ２与血管环孵浴１５ｍｉｎ。用ＮＥ使血管环收缩,再用Ａｃｈ使其舒张,计算最大舒张百分比。结果:损伤模型组中血管环在孵浴Ｈ２Ｏ２后对Ａｃｈ引起的内皮依赖性舒张作用比孵浴前明显减弱(Ｐ＜０．０１),而辛伐他汀组在孵浴Ｈ２Ｏ２前后血管环对Ａｃｈ引起的内皮依赖性舒张作用则无明显差异(Ｐ＞０．０５)。结论:５ ｍｍｏｌ Ｈ２Ｏ２能使血管环的内皮依赖性舒张作用明显减弱,而辛伐他汀能明显抵抗这种减弱作用。
Objective: To investigate the effect of simvastatin on H2O2-induced acute injury of arterial endothelial cells. Methods: Thoracic aorta of SD rats was made into 3 ~ 4mm vascular rings. The injury model group: with 5mmolH2O2 and vascular ring incubation for 15min; Simvastatin group: first with 50μmol simvastatin and vascular ring incubation 25min, rinsed, then 5mmol H2O2 and vascular ring incubation 15min. Vasoconstriction of the ring with NE, and then Ach to relax, calculate the maximum percentage of diastolic. Results: Endothelium-dependent vasodilation induced by Ach in the vascular rings in the model group was significantly weaker than that before the incubation (P <0.01), but not in the simvastatin group Induced endothelium-dependent vasodilation was no significant difference (P> 0.05). Conclusions: Endothelium-dependent vasodilation of vascular rings was significantly attenuated by 5 mmol H2O2, whereas simvastatin significantly counteracted this attenuating effect.