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目的:研究随着时间延长,DAPI标记间充质干细胞(MSCs)的标记率变化。方法:分离培养纯化成年大鼠骨髓MSCs,DAPI标记,不同时点荧光显微镜观察,计算MSCs中DAPI阳性率。将标记的MSCs移植到缺血下肢模型动物骨骼肌中,不同时点取材,冰冻切片,观察移植细胞。结果:标记当天,荧光镜下MSCs胞核呈明亮蓝色荧光,标记率接近100%,随着培养时间延长,标记率逐渐下降。移植1周和2周组织切片中,可见密集移植MSCs,3周以后,可检出的DAPI阳性细胞明显减少。结论:DAPI短期标记细胞效率很高,但标记率随着时间延长而迅速下降。
Objective: To study the changes of labeling rate of DAPI-labeled mesenchymal stem cells (MSCs) with time prolonging. Methods: Bone marrow MSCs and DAPI markers were isolated and purified from adult rats. Fluorescence microscopy was performed at different time points to calculate the positive rate of DAPI in MSCs. The labeled MSCs were transplanted into the skeletal muscle of ischemic lower extremity model, drawn at different time points, frozen section, observe the transplanted cells. Results: On the day of labeling, the fluorescence of MSCs under fluorescent microscope showed bright blue fluorescence and the labeling rate was close to 100%. With the extension of culture time, the labeling rate decreased gradually. Mice were transplanted for 1 and 2 weeks after transplantation. DMSI-positive cells could be detected after 3 weeks. Conclusion: DAPI short-term labeling of cells with high efficiency, but the labeling rate decreased rapidly with time.