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【目的】分析大麦对新型除草剂丙酯草醚的生理反应,研究该除草剂处理对大麦幼苗的生理指标及根系细胞亚显微结构、有丝分裂的影响,为明确丙酯草醚在大麦田的使用安全性和作用机理奠定理论基础。【方法】在大麦四叶期喷施不同浓度丙酯草醚,分析处理后乙酰乳酸合成酶(ALS)活性、蛋白含量、叶绿素荧光参数、叶绿素含量、光合速率的变化趋势,并研究100 mg·L-1浓度丙酯草醚对根尖细胞发育的影响。【结果】田间推荐浓度100 mg·L-1丙酯草醚在处理初期对大麦幼苗的影响较小,但随处理时间延长,该浓度处理对大麦叶片ALS活性、蛋白含量、光合系统各项参数的抑制作用加剧,并造成根尖细胞液泡体积减小、数量减少、微粒体数量增多、有丝分裂指数降低等现象。500、1 000 mg·L-1浓度处理在不同时期对大麦各项生理指标均表现出显著的抑制作用。【结论】大麦对100 mg·L-1丙酯草醚在处理后期反应较为敏感,500、1 000 mg·L-1浓度处理对苗期大麦不安全,光合系统受抑制及线粒体中微粒体的变化可能与丙酯草醚的作用机理有关。
【Objective】 The objective of this study was to analyze the physiological response of barley to the new herbicide propyl oxalate and to study the effects of herbicide treatment on the physiological indexes of barley seedlings and the subcellular microstructure and mitosis of root cells. The use of safety and mechanism of action to lay the theoretical foundation. 【Method】 The changes of ALS activity, protein content, chlorophyll fluorescence parameters, chlorophyll content and photosynthetic rate in four-leaf stage of barley were sprayed with different concentrations of propyl gallate. The effects of 100 mg · Effect of Propyl Ester at L-1 Concentration on the Development of Root Tip Cells. 【Result】 The results showed that the recommended concentration of 100 mg · L -1 propyl oxalate in the field had little effect on the seedling emergence of barley at the initial treatment stage. However, with the prolongation of treatment time, the concentration of ALS activity, protein content, photosynthetic system parameters Of the inhibition increased, and caused the root tip cell vacuole volume decreases, the number decreased, increased the number of microsomes, mitotic index decreased and so on. The concentrations of 500 and 1 000 mg · L-1 significantly inhibited the physiological indexes of barley at different stages. 【Conclusion】 Barley is sensitive to 100 mg · L-1 propylthio-propargyl ether at the late stage of treatment. The treatment with 500 and 1 000 mg · L-1 of barley is not safe for barley at seedling stage, and the photosynthetic system is inhibited and the microsomal Changes may be related to the role of propyl fenbufen mechanism.