Effects of β-aescin on apoptosis induced by transient focal cerebral ischemia in rats

来源 :Acta Pharmacologica Sinica | 被引量 : 0次 | 上传用户:fengyuguohou2009
下载到本地 , 更方便阅读
声明 : 本文档内容版权归属内容提供方 , 如果您对本文有版权争议 , 可与客服联系进行内容授权或下架
论文部分内容阅读
ABM: To investigate the effects ofβ-aescin on apoptosis induced by transient focal brain ischemia in rats. METHODS: Rats were pretreated with β-aescin for 7 d and then subjected to brain ischemia/reperfusion (I/R) injury induced by a middle cerebral artery occlusion. After 2 h ischemia and 24 h reperfusion, Hematoxylin-Eosin (HE) staining, in situ end-labeling of nuclear DNA fragmentation (TUNEL) were employed to determine the level of apoptosis. The expressions of caspase-3 and Bcl-2 in the cortex were determined by immunohistochemistry and Western blot. The release of cytochrome c was analyzed by Western blot. RESULTS: The increased numbers of HE- and TUNEL-positive staining cells were significantly observed at 24 h after reperfusion. The immunoreactivity was inhibited by β-aescin (30, 60 mg/kg) (P<0.01 or P<0.05 vs vehicle-treated). After cerebral I/R, cytochrome c was released into the cytosol and caspase-3 was activated, whereas Bcl-2 expression was inhibited. β-Aescin (30, 60 mg/kg) markedly in ABT: To investigate the effects of β-aescin on apoptosis induced by transient focal brain ischemia in rats. METHODS: Rats were pretreated with β-aescin for 7 d and then subjected to brain ischemia / reperfusion (I / R) injury induced by a middle Cerebral occlusion occlusion. After 2 h ischemia and 24 h reperfusion, Hematoxylin-Eosin (HE) staining, in situ end-labeling of nuclear DNA fragmentation (TUNEL) were employed to determine the level of apoptosis. The expressions of caspase-3 and Bcl -2 in the cortex were determined by immunohistochemistry and Western blot. RESULTS: The increased numbers of HE- and TUNEL-positive staining cells were significantly observed at 24 h after reperfusion. The immunoreactivity was inhibited by β-aescin (30, 60 mg / kg) (P <0.01 or P <0.05 vs vehicle-treated). After cerebral I / R, cytochrome c was released into the cytosol and caspase-3 was activated, 2 expression was inhibited. Β-Aescin (30, 60 mg / kg) markedly in
其他文献
目的探讨慢性丙型肝炎肝组织病变与肝细胞凋亡的相互关系.方法采用免疫组化方法对比检测了30例慢性丙型肝炎患者肝组织中的Fas抗原(FasAg)和Fas配体(FasL)的表达.结果FasAg的表达主要在肝细胞浆,尤以邻近“
一、科学发展观回答了新世纪新阶段实践提出的新课题针对我国发展中长期存在重视经济数量增长、经济发展方式陈旧和质量效益较低的问题,党中央提出,科学发展观的第一要义是发
吴延安做出全面部署,要求各公安消防部门加强基层建设的紧迫感和责任感;以“161”工程为抓手,坚持科学发展观,全面、协调地加强消防工作,进一步提升上海的防灾能力和人的消防
男11例,女2例,年龄18~69岁,平均41岁。修补后再穿孔时间:5例分别为术后3~8天,8例为术后8个月至3年。溃疡及穿孔情况:十二指肠溃疡穿孔9例,胃溃疡穿孔4例,其中1例胃溃疡穿孔再
思想政治工作是我们一切工作的生命线。在新的历史条件下,国有林区生产经营方式发生着深刻的变化,思想政治工作为林区建设提供了有效保证。随着林区的改革和发展进一步深入,
在中国思想文化史上,南宋是一重要时期。军事上的妥协与政治上的偏安,不仅并未影响宋文化的发展和繁荣,而且促使其形成独特的风貌。从哲学上看,北宋诸多学派并立的格局发生了重要的变化,在北宋时影响极大的王安石新学与三苏蜀学渐趋冷落,二程一系的理学得到空前的发展,成为占据主流地位的学派。从文学上看,在北宋后期王安石、苏轼、黄庭坚、陈师道诸大家辞世后,诗坛经过数十年的沉寂,重又焕发出生机,以陆游、范成大、杨万里为代表,宋诗出现了中兴复盛的新局面。从表面上看,思想史和文学史的走向及其特征的形成,各有其自身的原因,但深一
2004年6月17日上午,省林业局副局长杨新世、省森林公安局局长李桂征及全省8个市(廊坊、衡水、沧州除外)林业局的副局长、森林公安局长、省直属单位负责人和平山、易县等部门
噩耗传来2004年10月1日下午5时许,案头电话的鸣声突然响起,是任老先生的次子琪新打来的,说他的父亲正在曙光医院重症病房抢救,恐怕不行了,“叔叔是我父亲的挚交,望来见最后
从与奶奶相依为命,一条裤子正反穿到住着近100平方米的楼房,用着品牌电器,退休工资超过了2000元……作为全国劳动模范的他还经常到北戴河、青岛等地疗养,顾文平“很满足”。
采用ABAQUS有限元软件,对型钢再生混凝土框架-再生砌块填充墙结构抗震性能进行非线性分析,并与试验结果相对比,验证了所建模型的合理性。进而对该结构进行参数分析,研究了再