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目的探讨肺腺癌及肺鳞癌细胞遗传物质的改变与临床特征的关系。方法应用多重荧光原位杂交(M-FISH)和比较基因组杂交(CGH)技术,检测肺腺癌及肺鳞癌细胞株和肺癌组织中的DNA。结果在160例肺癌标本基因组中,M-FISH显示5、6、11、12、17号染色体频繁参与染色体间的易位;CGH显示最常见的扩增区域是1q,2p,3q,5p,5q,7p,8q,11q,12q,14q,16p,17p,19p,20q,21q,22q,最常见的缺失区域是2q,3p,4p,5q,7q,8p,9p,13q,14q,17p。结论M-FISH和CGH技术是研究肺癌基因组变化强有力的工具,该实验中发现的基因改变可能代表了与肺腺癌及肺鳞癌特有的候选基因。
Objective To investigate the relationship between the genetic changes and clinical features of lung adenocarcinoma and lung squamous cell carcinoma. Methods Multiplex fluorescence in situ hybridization (M-FISH) and comparative genomic hybridization (CGH) techniques were used to detect DNA in lung adenocarcinoma, lung squamous cell carcinoma and lung cancer tissues. Results In the genome of 160 lung cancer specimens, M-FISH showed that chromosomes 5, 6, 11, 12, and 17 were frequently involved in translocations between chromosomes; CGH showed the most common amplified regions were 1q, 2p, 3q, 5p, 5q The most common deletion regions are 7p, 8q, 11q, 12q, 14q, 16p, 17p, 19p, 20q, 21q, 22q, 2q, 3p, 4p, 5q, 7q, 8p, 9p, 13q, 14q, 17p. Conclusions M-FISH and CGH technology are powerful tools for the study of lung cancer genome changes. The genetic changes found in this experiment may represent candidate genes unique to lung adenocarcinoma and lung squamous cell carcinoma.