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ｇｐ１３０是一种分子量为１３０ｋＤ的细胞膜糖蛋白，是ＩＬ－６等多种细胞因子共用的信号传递分子。本研究将含ｇｐ１３０胞外区全长的可溶性片段的基因（ｓｇｐ１３０ｃＤＮＡ）构建到痘苗病毒载体中，并与野生型痘苗病毒在ＴＫ－１４３细胞中发生同源重组，用ＢｕｄＲ和Ｘ－ｇａｌ双重选择，得到带有人ｓｇｐ１３０的重组痘苗病毒（Ｖｓｇｐ１３０）。采用ＩＤＡ和ＷｅｓｔｅｒｎＢｌｏｔｉｎｇ法证实：感染Ｖｓｇｐ１３０的ＴＫ－１４３细胞上清中有较强的ｓｇｐ１３０表达，表达产物分子量为１００ｋＤ左右。
gp130 is a cell membrane glycoprotein with a molecular weight of 130 kD and is a signaling molecule shared by many cytokines such as IL-6. In this study, we constructed the full-length soluble fragment of gp130 gene (sgp130cDNA) into vaccinia virus and homologously recombined with wild-type vaccinia virus in TK-143 cells. Double-selection with BudR and X-gal , Resulting in recombinant vaccinia virus with human sgp130 (Vsgp130). The results of IDA and Western Bloting showed that sgp130 was expressed in the supernatant of TK-143 cells infected with Vsgp130, and the molecular weight of the expressed product was about 100 kD.