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目的 探讨 9号染色体的改变在皮肤肿瘤发生过程中的作用。 方法 应用 D9S319(9p21)和 D9S299(9q22.2-q22.3)2个座位微卫星 DNA多态标记，对基底细胞癌、鳞状细胞癌和 Bowen病进行了杂合性丢失分析。 结果 在可提供信息的 10例基底细胞癌、 19例鳞状细胞癌和 4例 Bowen病中，均未观察到 D9S319(9p21)杂合性丢失的存在； 10例基底细胞癌中，有 2例在 9q22.2-q22.3(D9S299)位点出现杂合性丢失；因此，在 9q可能存在具有潜力的抑癌基因。而在 21例鳞状细胞癌及 4例 Bowen病中，均未观察到 D9S299杂合性丢失的存在。结论 9q22.2-q22.3可能存在具有潜力的抑癌基因，其缺失在基底细胞癌的发生中起一定作用。
Objective To investigate the role of chromosome 9 changes in the development of skin tumors. Methods The microsatellite DNA polymorphism markers D9S319 (9p21) and D9S299 (9q22.2-q22.3) were used to analyze the loss of heterozygosity in basal cell carcinoma, squamous cell carcinoma and Bowen’s disease. Results No loss of heterozygosity for D9S319 (9p21) was observed in 10 basal cell carcinomas, 19 squamous cell carcinomas, and 4 Bowen’s disease cases, and 2 of 10 basal cell carcinomas Loss of heterozygosity at the 9q22.2-q22.3 (D9S299) locus; therefore, potential tumor suppressor genes may exist at 9q. However, in 21 cases of squamous cell carcinoma and 4 cases of Bowen’s disease, no loss of heterozygosity of D9S299 was observed. Conclusion 9q22.2-q22.3 may have potential tumor suppressor gene, its deletion plays a role in the occurrence of basal cell carcinoma.