我国双孢蘑菇(Agaricus bisporus)年产量占全世界食用菌总产量的70%,但是它不耐高温,病毒病常常使栽培者损失惨重。而大肥菇(Agoricusbitorq(?)is)不但耐高温,而且能抗双孢蘑菇病毒的侵杂。为了克服种间不亲和障碍,培育耐高温抗病毒的蘑菇新品种,我们开展了双孢蘑菇与大肥菇的原生质体融合研究。本文主要报告双孢蘑菇与大肥菇原生质体制备条件研究结果。 Agaricus bisporus produces about 70% of the world’s total edible mushrooms per year, but it does not tolerate high temperatures and viral diseases often cause grower losses. Agrofusus mushroom (Agoricus bitorq (?) Is) is not only high temperature, but also resistant to Agaricus bisporus viruses. In order to overcome intersexual incompatibility disorders and cultivate new varieties of mushrooms with high temperature and antiviral activity, we carried out the protoplast fusion study of Agaricus bisporus and Agaricus bisporus. This article mainly reports Agaricus bisporus Protoplast preparation conditions and research results.