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目的探讨胎猪皮肤前体组织异种移植模型及移植后发育过程。方法取精确胎龄为56d的胎猪皮肤前体组织,分组进行移植,①背部创面模型组:制备成微粒皮后移植到BALB/c裸鼠背部创面,以人尸体皮覆盖;背部皮下模型组:制备成小张邮票皮,植入裸鼠背部皮下;耳廓皮下模型组:制备成微粒皮植入裸鼠耳廓皮下。各组于移植后观察其生长发育特性,并于移植后6周和12周取材行HE染色观察其组织结构形态。新生组织块大小采用双样本t检验。结果各模型的胎猪皮肤前体组织移植后均能存活并继续生长,背部创面模型组生长能力显著大于耳廓皮下模型组,新生皮肤组织均具有真皮和表皮,耳廓皮下模型的真皮附属器仅见毛囊(毛发),偶见皮脂腺,未见汗腺,但是背部创面模型组及背部皮下模型组不仅能发育成毛囊(毛发)、还见大量皮脂腺和汗腺。结论背部创面移植模型最适合于胎猪皮肤前体组织异种移植及移植后发育的研究。
OBJECTIVE To investigate the model of preimplantation tissue of fetal pigs and the developmental process after transplantation. Methods Tissue of precancerous tissues of fetal piglets with a precise gestational age of 56 days was grafted and divided into groups. ① The model group of the back wounds was made into microsomes and then transplanted into the back wounds of BALB / c nude mice and covered with human cadaver skin. : Prepared into Zhang stamps skin, implanted subcutaneously in the back of nude mice; auricle subcutaneous model group: prepared into micro-skin graft implanted in the auricle of the mouse subcutaneous. The growth and development of each group were observed after transplantation, and the tissue morphology was observed by HE staining at 6 and 12 weeks after transplantation. Neonatal tissue block size using two-sample t-test. Results All the models of fetal skin precursor tissue could survive and continue to grow after transplantation. The growth ability of the model group was significantly greater than that of the auricle subcutaneous model group. The newborn skin tissue had both dermis and epidermis. Seen only hair follicles (hair), occasionally sebaceous glands, no sweat glands, but the back of the wound model group and the back of the subcutaneous model group can not only develop hair follicles (hair), also see a large number of sebaceous glands and sweat glands. Conclusion The dorsal wound transplantation model is the most suitable for the study of fetal rat skin precursor tissue xenotransplantation and post-transplantation development.