Ｈｃａ－Ｆ（Ｆ）和Ｈｃａ－Ｐ（Ｐ）是从小鼠肝癌中分离出来的只经淋巴道转移的同源而具有不同转移能力的两株克隆细胞系，Ｆ细胞转移能力明显高于Ｐ细胞。经淋巴结冰冻切片粘附的动态观察发现：Ｆ细胞粘附率明显高于Ｐ细胞，且高峰提前２４ｈ，粘附时间亦较长。光镜下早期两株瘤细胞均粘附于网状纤维支架上。在瘤细胞聚集区网状纤维连续性中断甚至完全消失。电镜下见Ｆ细胞绒毛较多，并伸出勾状突起插入细胞外基质中，突起周围见空隙存在，使基质连续性中断。Ｐ细胞虽亦与基质粘附，但突起少而不明显未见插入细胞周围基质现象。该结果提示：对淋巴结内无定形基质粘附的特性及其牢固程度很可能是形成淋巴结转移灶的基础；这给阻断癌细胞转移提供理论基础 Hca-F (F) and Hca-P (P) are two clonal cell lines with different metastatic potential only lymphatic metastasis derived from mouse hepatocarcinoma. The metastatic ability of F cells is obviously higher than that of P cells . Dynamic observation of lymph node frozen sections adhesion: F cell adhesion rate was significantly higher than P cells, and the peak early 24h, adhesion time is longer. Light microscope early two tumor cells were adhered to the mesh fiber scaffold. The continuity of reticular fibers in tumor cell aggregates disrupted or even completely disappeared. Under electron microscopy, F cells have more villi and extend the hook-shaped protrusions into the extracellular matrix. The presence of voids in the periphery of the protuberances interrupts the continuity of the matrix. P cells, although also with the matrix adhesion, but the prominence of the prominence, but no obvious phenomenon was not inserted into the surrounding matrix cells. The results suggest that the characteristics of adherence to the amorphous matrix in the lymph nodes and their robustness are likely to underlie the formation of lymph node metastases; this provides the rationale for blocking cancer cell metastasis