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目的 研究单侧坐骨神经结扎对大鼠腰 4～ 5脊髓节段和相应的背根神经节 (DRGs)内VGluT1样免疫阳性反应产物表达的影响以及VGluT1通过轴浆流向外周转运的情况。 方法 采用免疫组织化学方法观察单侧坐骨神经结扎后不同时间内腰 4～ 5脊髓节段、DRGs和结扎部位的近、远侧端神经干内VGLuT1样免疫阳性反应强度的变化。结果 (1)坐骨神经结扎后第 1和第 2天 ,VGluT1样免疫阳性产物在结扎的同侧腰 4～ 5脊髓节段和相应节段的DRGs内未检测到明显变化 ;但自术后第 4天开始 ,可观察到VGluT1样免疫阳性产物的表达在上述部位逐渐减弱 ;VGluT1样免疫阳性产物表达的降低在上述部位所出现的时间和程度相平行。 (2 )结扎后第 1天即可观察到VGluT1样免疫阳性产物在坐骨神经结扎部位近侧端的表达有所升高 ,但自术后第 4天开始逐渐降低 ;而VGluT1样免疫阳性产物在坐骨神经结扎部位远侧端的表达自结扎后第 1天起就逐渐降低 ,至第 4周时已完全消失。结论 (1)DRG神经元合成VGluT1,并通过轴浆流将VGLluT1向中枢突和周围突运输 ,故腰髓内部分VGluT1样阳性末梢起源于DRG神经元 ;(2 )外周神经的损伤很易影响到DRG神经元内VGluT1的合成
Objective To investigate the effect of unilateral sciatic nerve ligation on the expression of VGluT1-like immunoreactive products in the spinal cord segments 4 ~ 5 and corresponding DRGs in rats and the transport of VGluT1 to the periphery through axoplasmic flow. Methods Immunohistochemistry was used to observe the changes of VGLuT1-like immunoreactivity in the proximal and distal trunk of DRGs and ligation sites at 4 ~ 5 spinal cord segments at different time points after unilateral sciatic nerve ligation. Results (1) No obvious changes of VGluT1-like immunopositive products were found in DRGs of 4 ~ 5 spinal cord segments and corresponding segments of the ipsilateral lumbar ligation on the 1st and 2nd day after sciatic nerve ligation. However, From the beginning of the day, it was observed that the expression of VGluT1-like immunoreactive products gradually diminished at these sites; the decrease in the expression of VGluT1-like immunopositive products paralleled the timing and extent of appearance of these sites. (2) The expression of VGluT1-like immunopositive products at the proximal end of the sciatic nerve ligation site was observed on the first day after ligation but gradually decreased from the fourth day after the operation. However, VGluT1-like immunopositive products were observed in the sciatic nerve ligation The distal part of the site of expression from the first day after the ligation gradually decreased until the 4th week has completely disappeared. Conclusion (1) DRG neurons synthesize VGluT1 and transport VGLluT1 to the central and peripheral axons through axoplasmic flow. Therefore, some VGluT1-like positive terminals in the lumbar spinal cord originate from DRG neurons. (2) Peripheral nerve injury is easily influenced Synthesis of VGluT1 into DRG neurons