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本研究以134份陆地棉栽培种为试验材料,测定其在0.3%盐浓度(质量分数)下的出苗率,并使用74对SSR引物对这些材料进行基因组变异扫描。利用Structure2.3.4软件分析该自然群体的遗传结构,在此基础上采用Tassel2.1软件对耐盐性状与SSR分子标记进行关联分析,寻找与棉花耐盐性状相关的分子标记。研究结果表明:(1)134份陆地棉栽培种的出苗率呈极显著差异,并筛选出27个盐敏感材料和10个耐盐材料。(2)74个SSR分子标记共检测出148个多态性位点,涉及246个等位变异,变异范围为2~7,平均每个标记3.32个;基因多样性指数变异范围为0.0295~0.4959,平均值为0.2897;SSR分子标记多态性信息含量(PIC)变幅为0.0290~0.3729,平均值为0.2381。(3)通过群体结构分析,将该自然群体划分2个亚群体,分别包含89份和45份材料。(4)关联分析共发现8个与棉花耐盐性状相关的SSR分子标记位点,表型变异解释率变幅为2.91%~7.82%,平均值为4.32%。此研究结果可以为棉花耐盐性状分子标记辅助选择育种提供参考。
In this study, 134 cultivated G. hirsutum cultivars were used as experimental material to determine the seedling emergence rate at 0.3% salt concentration (mass fraction), and 74 pairs of SSR primers were used to perform genome mutation scanning on these materials. The genetic structure of this natural population was analyzed using Structure2.3.4 software. Based on this, Tassel2.1 software was used to correlate salt tolerance traits with SSR molecular markers and find out molecular markers related to salt tolerance traits in cotton. The results showed that: (1) The emergence rate of 134 cultivars of G. hirsutum was significantly different, and 27 salt-sensitive materials and 10 salt-tolerant materials were screened. (2) A total of 148 polymorphic loci were detected in 74 SSR markers, involving 246 alleles, ranging from 2 to 7 with an average of 3.32 for each marker. The range of genetic diversity index ranged from 0.0295 to 0.4959 , With an average of 0.2897. The polymorphism information content (PIC) of SSR ranged from 0.0290 to 0.3729 with an average of 0.2381. (3) According to the analysis of group structure, this natural population is divided into two subgroups, each containing 89 and 45 materials. (4) Correlation analysis revealed eight SSR loci related to salt tolerance in cotton. The explained rate of phenotypic variation ranged from 2.91% to 7.82% with an average value of 4.32%. The results of this study can provide a reference for molecular marker-assisted selection breeding of salt tolerant traits in cotton.