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通过口服米非司酮 ( RU486)改变子宫内膜的发育进程 ,观察内膜 H OXA11基因表达量的变化 ,以及与孕酮受体 ( PR)和白血病抑制因子 ( L IF)表达的相互关系。 13例月经周期正常志愿者观察两个周期。于对照周期的分泌中期取内膜。实验周期排卵前或后一次性口服 RU48610 mg,分泌中期取内膜 ,用原位杂交和免疫组织化学方法检测H OXA11、PR及 LIF的表达。结果 :对照周期内膜腺上皮 H OXA11和 PR呈阴性或弱阳性表达。服药后 ,腺上皮 H OXA11和 PR表达强度大多增加 ,而 LIF的表达强度大多下降 ;基质细胞的表达量变化无明显规律。提示 H OXA11基因的表达与孕激素及其受体密切相关 ;分泌中期腺上皮 H OX A11和 PR表达下降或消失是内膜分化成熟的重要标志 ;也是 L IF分泌达高峰的前提。
The change of endometrial H OXA11 gene expression and the relationship with the expression of progesterone receptor (PR) and leukemia inhibitory factor (L IF) were observed by changing the course of endometrium development by oral injections of mifepristone (RU486). Thirteen normal menstrual cycle volunteers observed two cycles. In the control period during the secretion of endometrial take. RU48610 mg was administered orally once or orally after the test period, and the endometrium was secreted. The expression of H OXA11, PR and LIF were detected by in situ hybridization and immunohistochemistry. Results: H OXA11 and PR were negative or weakly positive during the control period. After taking the medicine, the intensity of expression of H OXA11 and PR in the glandular epithelium mostly increased, but the expression intensity of LIF mostly decreased. The expression of stromal cells did not change obviously. It is suggested that the expression of H OXA11 gene is closely related to progesterone and its receptor. The decrease or disappearance of H OX A11 and PR expression in the middle stage of secretory gland is an important marker of intima maturation and the premise of the peak of L IF secretion.