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利用Ta1小麦 Ms2 创建改良小麦面包品质的优质群体,采用SDS-PAGE法对其2次互交轮回群体C2的HMW-GS组成进行了分析.结果表明:在供试的193个样品中各HMW-GS及其组成模式的频率不尽相同,Glu-A1、Glu-B1和Glu-D1位点上产生频率最高的亚基分别是1、14+15和2+12,各为54.40%、35.75%和60.10%,优质亚基5+10的频率为17.6%; null、14+15、2+12 模式产生频率最高,为13.47%,并有 14+15,5+10 的优质亚基聚合体出现,占5.2%;该群体也产生了亲本不具有的13、16、22亚基及19种新的HMW-GS组成模式.说明利用Ta1小麦轮回选择技术是创造新亚基类型的一个有效途径.
In this study, the HMW-GS composition of C2 crosses of two reciprocal crosses was analyzed by SDS-PAGE using Ta1 wheat Ms2.The results showed that among the 193 samples tested, HMW- The frequency of GS and its compositional patterns were different. The highest frequency of subunits of Glu-A1, Glu-B1 and Glu-D1 were 1,14 + 15 and 2 + 12 respectively, which were 54.40% and 35.75% And 60.10%, respectively. The frequency of high quality subunit 5 + 10 was 17.6%. The highest frequency of null, 14 + 15,2 + 12 was 13.47%, and 14 + 15, 5 + 10 high subunit polymers appeared , Accounting for 5.2% .This group also produced the 13,16,22 subunits and 19 new HMW-GS composition patterns that the parent did not have, which indicated that Ta1 wheat cycle selection was an effective way to create new subunit types.