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目的研究抗氧化剂２，３叔丁基４羟基茴香醚（ＢＨＡ）对黄曲霉毒素（ＡＦＢ１）染毒的乙型肝炎病毒（ＨＢＶ）转基因小鼠肝肿瘤发生的防护作用。方法对ＡＦＢ１染毒和未染毒的４９只转基因小鼠与４８只非转基因小鼠，测定肝醌还原酶（ＱＲ）和谷胱甘肽Ｓ转移酶（ＧＳＴ）活性、肝丙二醛（ＭＤＡ）含量、肝氧自由基浓度（ＯＦＲ）。结果染毒的转基因小鼠ＢＨＡ组肝腺瘤发生率１７％，癌变率０，显著低于普通饲料组的肝腺瘤发生率（６７％）和癌变率（２２％）。与普通饲料组比较，ＢＨＡ使肝ＯＦＲ和ＭＤＡ含量显著降低；ＢＨＡ组肝ＱＲ和ＧＳＴ活性平均升高３～７倍。结论ＢＨＡ能显著诱导肝脏生物转化Ⅱ相酶活性，清除氧自由基，抑制变异肝细胞生长，可能与抑制或延缓小鼠肝癌的发生、发展进程有关
Objective To study the protective effect of antioxidant 2,3tert-butyl4-hydroxyanisole (BHA) on hepatic tumors of hepatitis B virus (HBV) transgenic mice exposed to aflatoxin (AFB1). Methods The activity of hepatic quinone reductase (QR) and glutathione S transferase (GST) and hepatic malondialdehyde (MDA) were determined in 49 transgenic mice and 48 non-transgenic mice exposed to and not exposed to AFB1. )Content, hepatic oxygen free radical concentration (OFR). Results The incidence of hepatic adenoma in the BHA group was 17% and the cancerous rate was 0, which was significantly lower than that of the normal feed group (67%) and cancerous rate (22%). Compared with the normal feed group, BHA significantly decreased liver OFR and MDA content; BHA group liver Qi and GST activity increased 3 to 7 times. Conclusion BHA can induce phase II biotransformation of liver enzymes, scavenge oxygen free radicals, and inhibit the growth of mutant hepatocytes, which may be related to the inhibition or delay of the occurrence and development of liver cancer in mice.