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目的:评价何首乌有效成分对急性酒精性肝损伤的预防作用。方法:采用乙醇灌胃法复制急性酒精性肝损伤小鼠模型,爬杆实验检测小鼠的清醒程度,测定血清和肝脏生化指标、病理组织HE染色透射电镜。结果:与模型组比较,不同剂量药物组对小鼠肝损伤的预防作用存在差异显著。小鼠血清中谷丙转氨酶(ALT)、谷丙转氨酶(AST)活性、甘油三酯(TG)、总蛋白(TP)含量有显著降低。通过病理组织HE染色和透射电镜观察何首乌有效成分可显著减少肝细胞内脂滴的聚集,和检测指标一致。结论:何首乌有效成分对急性酒精性肝损伤具有预防作用。
Objective: To evaluate the preventive effect of the active ingredients of Polygonum multiflorum on acute alcoholic liver injury. Methods: The mice model of acute alcoholic liver injury was duplicated by ethanol gavage. The mice were awakened by climbing pole test. The biochemical indexes of serum and liver were measured. The pathological tissues were stained with HE for transmission electron microscopy. Results: Compared with the model group, different doses of drug group on the prevention of liver injury in mice there are significant differences. The levels of ALT, AST, triglyceride (TG) and total protein (TP) in the serum of mice were significantly decreased. The pathological HE staining and transmission electron microscopy of Polygonum multiflorum active ingredients can significantly reduce the accumulation of lipid droplets within the hepatocytes, and the detection of the same indicators. Conclusion: Polygonum multiflorum active ingredients can prevent acute alcoholic liver injury.