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研究了聚合氯化铝(PACl)对序批式生物膜滤池(SBBF)处理生活污水的强化作用,包括去除效能的影响和基于454焦磷酸测序的微生物多样性分析.研究结果表明:PACl投加可使得SBBF获得满意的碳氮磷去除效果,同时高通量测序结果有助于认识铝盐对废水生物处理系统微生物的影响;50mg·L~(-1)的PACl投加可以使出水的总磷(TP)去除量从48.7%显著提高至68.2%,TP浓度低于0.5mg·L~(-1),对氮和化学需氧量(COD)去除的影响较小;454焦磷酸测序分析揭示铝胁迫下SBBF的微生物的多样性和丰度;铝盐投加可以使得浮游菌属(Planctomyces)、消化链球菌属(Peptostreptococcaceae)和嗜冷菌属(Psychrobacter)的丰度减少,假单胞菌属(Pseudomonas)和梭状芽孢杆菌属(Clostridium)的丰度增加,而不动杆菌属包括tjernbergiae不动杆菌(Acinetobacter tjernbergiae)和鲁菲不动杆菌(Acinetobacter lwoffii)一直处于主导地位.
The effect of polyaluminum chloride (PACl) on domestic wastewater treatment by sequencing batch biofilm filter (SBBF) was studied, including the removal efficiency and microbial diversity analysis based on 454 pyrosequencing.Results show that PACl Addition of SBR can make SBBF obtain satisfactory removal efficiency of carbon, nitrogen and phosphorus, at the same time, the results of high-throughput sequencing help to understand the influence of aluminum salt on the microorganism of wastewater biological treatment system; PACl of 50mg · L -1 can make effluent TP removal was significantly increased from 48.7% to 68.2%, TP was less than 0.5 mg · L -1, and had little effect on removal of nitrogen and chemical oxygen demand (COD). 454 pyrosequencing The analysis revealed the diversity and abundance of microorganisms in SBBF under aluminum stress. The addition of aluminum salt reduced the abundance of Planctomyces, Peptostreptococcaceae and Psychrobacter, The abundance of Pseudomonas and Clostridium is increasing, while Acinetobacter species including Acinetobacter tjernbergiae and Acinetobacter lwoffii have been dominant.