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采用常规方法对广东省番禺和五山两地种植的水稻内生放线菌进行分离、鉴定和分析 ,结果表明水稻内生放线菌多属于链霉菌属 (Streptomyces) ,其中灰褐类群链霉菌 (S .griseofuscus)的分离频率最高为 36 1%～ 6 9% ,是水稻植株中的优势内生放线菌类群。研究了内生放线菌在水稻植株各器官中的分布 ,结果表明根中内生放线菌的多样性高于茎叶。番禺地区种植的水稻中分离出的内生放线菌种类较多。从感病品种及生长不良水稻植株中分离出的内生放线菌种类比较丰富。通过回接分离试验及利用扫描电镜观察内生菌在植物体内分布发现 ,水稻优势内生放线菌回接无菌组培苗后 ,不仅能够定殖在水稻植株的根表和根内部 ,而且存在于茎杆和叶片中。通过平板颉抗及代谢物的活性测定试验 ,发现所分离的内生放线菌 5 0 %对水稻某些病原菌有颉抗活性 ,其中灰褐类群链霉菌的比例达到 5 5 4 % ,成为所分离的水稻内生放线菌类群中具有颉抗活性的最大群体。
Isolation, identification and analysis of endophytic actinomycetes from rice planted in Panyu and Wushan of Guangdong Province showed that most of the endophytic actinomycetes in rice belonged to Streptomyces, S griseofuscus, the highest frequency of 36 1% ~ 69%, was the dominant endophytic actinomycetes in rice plants. The distribution of endophytic actinomycetes in various organs of rice plants was studied. The results showed that the diversity of endophytic actinomycetes in roots was higher than that in stems and leaves. There are many endophytic actinomycetes isolated from rice planted in Panyu area. Endophytic actinomycetes isolated from susceptible and poorly-growing rice plants are abundant. Through the back-to-back separation experiment and observation of the distribution of endophytes in plants by scanning electron microscopy, it was found that the superior endophytic actinomycetes of rice could be colonized not only in the root and root of rice plants but also in the root of rice plants Exist in stems and leaves. Through the determination of antiplatelet activity and metabolite activity, it was found that 50% of the isolated endophytic actinomycetes had antagonistic activity against certain pathogenic bacteria in rice, in which the proportion of Streptomyces rubescens reached 55.4% Isolated rice endophytic actinomycetes in the group with the largest anti-Jie activity.