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依据菊花DUS测试指南中性状观测和分级的方法,记录了菊属、亚菊属31个物种叶片和花序的8个定性多态性状,测量了各物种叶片的14个形态指标,并将其转换为7个数量多态性状,分别基于定性多态性状代码和叶片数量多态性状数值利用聚类分析研究其亲缘关系,将2次聚类结果进行比较。结果表明,基于定性多态性状和叶片数量多态性状的聚类结果均可对菊花近缘属植物进行亲缘关系分析,并与前人利用分子标记的亲缘关系研究及植物学传统分类的研究结果一致,能够较准确地反映种间亲缘关系。而利用菊花DUS测试相关定性多态性状的信息进行亲缘关系研究,具有准确度高、采样简便、工作量小的优点。
According to the method of observing and classifying traits in the DUS test guideline of Chrysanthemum, 8 qualitative polymorphic traits of leaves and inflorescences of 31 species of Ju genus and Agarius were recorded, and 14 morphological indexes of leaves of each species were measured and converted 7 polymorphic traits were used to study the genetic relationship based on the polymorphic trait locus codes and the leaf number polymorphism traits, respectively. The results of the second clustering were compared. The results showed that the clustering results based on the polymorphism traits and leaf number polymorphism could be used to analyze the genetic relationship of the genus Chrysanthemum, and the genetic relationship with molecular markers and the traditional classification of botanicals Consistent, can more accurately reflect the kinship. However, using the information of qualitative polymorphism related to DUS test to study genetic relationship has the advantages of high accuracy, simple sampling and small workload.