论文部分内容阅读
目的：查明ＭＲＳＡ的流行情况并探索其防治措施。方法：采用Ｅｖｅｒｅｔ方法分离菌株，Ｋ－Ｂ法做９种抗生素的药物敏感试验，同时做了β－内酰胺酶产生试验和质粒消除实验。结果：共分离金黄色葡萄球菌５２株，其中ＭＲＳＡ１６例，检出率为３１％。药物敏感试验显示：ＭＲＳＡ比ＭＳＳＡ更具多重耐药性，并有极相似的耐药图谱，揭示该菌有在医院内流行的可能性。质粒消除实验证明：其抗性基因大都定位在质粒上，可在不同菌层中传播。结论：ＭＲＳＡ具有广泛的耐药谱，并可在医院内流行、传播。微生物实验室应对其进行监测。
Objective: To identify the prevalence of MRSA and explore its prevention and treatment measures. Methods: The Everest method was used to isolate the strains. K-B method was used to test the antibacterial activity of nine antibiotics. At the same time, β-lactamase production test and plasmid elimination test were performed. Results: A total of 52 Staphylococcus aureus isolates were isolated, of which MRSA was detected in 16 cases with a detection rate of 31%. Drug-susceptibility tests showed that MRSA was more multi-drug resistant than MSSA and had a very similar drug resistance profile, revealing the possibility of the bacteria being prevalent in hospitals. Plasmid elimination experiments show that most of their resistance genes are located on the plasmid and can spread in different layers of bacteria. Conclusion: MRSA has a wide range of drug resistance spectrum, and can be epidemic in hospitals. Microbiological laboratories should monitor it.