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目的:观察红花-甘草配伍对寒凝血瘀模型大鼠组织(心、肝、脾、肺、肾)中磷酸腺苷的代谢,探讨红花-甘草配伍改善寒凝血瘀模型大鼠组织中能量代谢的作用。方法:选用SD大鼠随机分为空白对照组、模型对照组、红花组和红花-甘草组,采用冷刺激的冷水冰浴方法制作寒凝血瘀大鼠模型。于造模成功后开始灌胃给药,每日1次,连续灌服15 d,于给药结束后12 h,取血及心、肝、脾、肺、肾各组织,测定各组大鼠的血液流变学参数,并采用HPLC法测定各组织中三磷酸腺苷(ATP)、二磷酸腺苷(ADP)、一磷酸腺苷(AMP)的含量。结果:与模型对照组比较,红花组和红花-甘草组的全血粘度、血浆黏度明显降低,ATP、ADP、AMP含量明显增高,且“红花-甘草”组优于红花组(P<0.05)。结论:红花对机体的能量代谢异常有改善作用;甘草能促进红花改善寒凝血瘀疾病的能量代谢障碍。
Objective: To observe the compatibility of Safflower-Glycyrrhiza uralensis (Glycyrrhiza uralensis) and Glycyrrhiza uralensis (Glycyrrhiza uralensis) on the metabolism of ATP in the tissues of heart, liver, spleen, lung and kidney in rats with cold and blood stasis syndrome The role of metabolism. Methods: SD rats were randomly divided into blank control group, model control group, safflower group and safflower - licorice group. Cold-blooded ice bath was used to make cold-blooded rat model. After the model was successfully established, gavage administration was started once a day for 15 days. The rats in the groups of heart, liver, spleen, lung and kidney were harvested at 12 h after the administration, The parameters of hemorheology were determined. The contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP) and adenosine monophosphate (AMP) were determined by HPLC. RESULTS: Compared with the model control group, the whole blood viscosity and plasma viscosity of safflower-safflower-licorice group were significantly decreased, the levels of ATP, ADP and AMP were significantly increased, and the “safflower-licorice” group was superior to Safflower- Group (P <0.05). Conclusion: Safflower can improve the energy metabolism of the body. Glycyrrhiza uralensis can promote the energy metabolism disorder of safflower to improve the coagulation and blood stasis disease.