V_2O_5/γ-Al_2O_3-TiO_2 catalysts were prepared by the mixing sol-gel and co-impregnation method. The performance of the catalysts for complete oxidation of ethanol was evaluated in a conventional fixed-bed quartz reactor. And the effects of support,preparation methods and vanadium content have been investigated. The results showed that 5% V_2O_5 catalyst supported on γ-Al_2O_3-TiO_2 possessed the best ethanol conversion under the considered temperature. This may be ascribed to the highly dispersible active component,mutual function between the active component and the carders. The nature of the best performance for 5%V/γ-Al_2O_3-TiO_2 catalyst may be related to the high V~(4+) amounts on the surface. And the surface V~(4+) species may play an important role in the formation of active site for the total ethanol oxidation.