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溶菌酶为正常机体非特异性免疫防御机制的组成部分之一。近来由于对非特异性免疫研究逐步深入,溶菌酶与临床多种疾病的密切关系逐步得到证实。它多用于白血病的分型,一些疾病的辅助诊断指标及某些炎症的治疗。本实验采取琼脂平板打孔法,测定了普通和无菌的六周龄豚鼠血清溶菌酶水平,探讨了两者免疫功能的区别。材料与方法 1.试剂: 磷酸盐缓冲液(M/15 pH6.4): 用pH6.4的PBS溶解琼脂粉配成1.1％
Lysozyme is one of the components of the normal body’s non-specific immune defense mechanism. Recently, due to the deepening of the research on non-specific immunity, the close relationship between lysozyme and various clinical diseases has been gradually confirmed. It is mostly used for the classification of leukemia, an auxiliary diagnostic indicator for some diseases and treatment of certain inflammations. In this experiment, serum levels of lysozyme in normal and sterile six-week-old guinea pigs were measured using agar plate punching method, and the differences in immune function between the two were discussed. Materials and Methods 1. Reagents: Phosphate buffer (M/15 pH6.4): 1.1% of agar powder dissolved in PBS pH 6.4