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目的建立RP-HPLC法同时测定利肝隆颗粒中芒柄花素、五味子醇甲、五味子甲素、五味子乙素4种成分的含量。方法采用Diamonsil C18色谱柱(200 mm×4.6 mm,5μm)进行分离;流动相:乙腈-甲醇(体积比为85∶15)(A)-体积分数为0.1%的磷酸水溶液(B),梯度洗脱,流速:1.0 m L·min-1,检测波长:254 nm,柱温:30℃。结果芒柄花素、五味子醇甲、五味子甲素、五味子乙素质量浓度分别在0.060 5~0.483 8 mg·L-1(r=0.999 5,n=5)、3.390~27.12 mg·L-1(r=0.999 7,n=5)、0.459 8~3.678 mg·L-1(r=0.999 7,n=5)、1.818~14.54 mg·L-1(r=0.999 6,n=5)内与峰面积呈良好的线性关系,方法的平均回收率分别为100.2%(RSD=1.1%,n=9)、99.1%(RSD=1.4%,n=9)、100.1%(RSD=1.4%,n=9)和99.4%(RSD=1.4%,n=9)。结论该方法可用于利肝隆颗粒的质量控制。
Objective To establish a RP-HPLC method for the simultaneous determination of four components of formononetin, schisandrin, schizandrin, and schisandrin B in Liganong granules. Methods The separation was performed on a Diamonsil C18 column (200 mm×4.6 mm, 5 μm); mobile phase: acetonitrile-methanol (85:15 volume ratio) (A)-0.1% aqueous solution of phosphoric acid (B), gradient washing Removal, flow rate: 1.0 m L·min-1, detection wavelength: 254 nm, column temperature: 30°C. Results The concentrations of formononetin, schisandrin, schizandrin, and schisandrin B were 0.060 5 to 0.483 8 mg·L-1 (r=0.999 5, n=5) and 3.390 to 27.12 mg·L-1, respectively. (r=0.999 7, n=5), 0.459 8 to 3.678 mg·L-1 (r=0.999 7, n=5), 1.818~14.54 mg·L-1 (r=0.999 6, n=5) There was a good linear relationship with peak area. The average recoveries of the method were 100.2% (RSD=1.1%, n=9), 99.1% (RSD=1.4%, n=9), and 100.1% (RSD=1.4%, respectively). n=9) and 99.4% (RSD=1.4%, n=9). Conclusion This method can be used to control the quality of Liganong Granules.