目的　构建 TGF- βR / Fc融合基因的真核表达载体 p Ig- TR ,使其在中国仓鼠肾细胞 (CHO)中呈分泌性表达 ,为肺间质纤维化基因治疗的研究奠定基础 .方法　采用RT- PCR方法从正常人肺组织中扩增出目的基因 TGF- βR c DNA的胞膜外部分 ,再将 TGF- βR c DNA片段亚克隆到p Ig 真核表达载体 ,该载体在其多克隆位点下游含有人Ig G1 Fc基因组片段 ,由此可形成 TGF-βR / Fc融合基因 ;采用脂质体转染技术转染 CHO细胞使其瞬时表达融合蛋白 ;应用细胞免疫荧光技术检测融合蛋白的表达 ;采用免疫沉淀的方法检测 CHO细胞培养上清内融合蛋白的表达 .结果　DNA测序结果在 Blast上作同源性比较证明插入片段就是TGF-βR c DNA的胞膜外部分 ,细胞免疫荧光染色呈阳性反应 ,CHO细胞培养上清内检测到融合蛋白的表达 .结论　p Ig- TR 真核表达载体构建成功 ,并能够在 CHO细胞中分泌性表达有活性的融合蛋白 TGF-βR / Fc Objective To construct the eukaryotic expression vector p Ig-TR of TGF-βR / Fc fusion gene and make it express in Chinese hamster kidney cells (CHO) for the study of gene therapy of pulmonary interstitial fibrosis.Methods RT-PCR method was used to amplify the extracellular portion of the TGF-βR c DNA of the target gene from normal human lung tissue. The TGF-βR c DNA fragment was subcloned into the p Ig eukaryotic expression vector, Downstream of the site contains human Ig G1 Fc genomic fragment, which can form TGF-βR / Fc fusion gene; transfected CHO cells by lipofection technique to transiently express the fusion protein; the use of immunofluorescence technology to detect fusion protein The expression of fusion protein in CHO cell culture supernatant was detected by immunoprecipitation.Results The sequence comparison of DNA sequencing showed that the inserted fragment was the extracellular part of TGF-βR c DNA, and the immunofluorescence staining The positive expression of fusion protein was detected in the culture supernatant of CHO cells.Conclusion The p Ig-TR eukaryotic expression vector was constructed successfully and secreted actively in CHO cells Proteins TGF-βR / Fc