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目的研究携带IL-7的新城疫病毒修饰的自体肿瘤疫苗的抗肝癌作用。方法鸡胚扩增新城疫病毒LX/(IL-7),体外感染Hepa1-6细胞,显微镜下观察LX/(IL-7)的裂解性特征,并采用ELISA的方法检测感染Hepa1-6细胞后IL-7的表达水平;将C57BL/6小鼠分为2组,每组5只,通过皮下接种5×106个Hepa1-6细胞建立小鼠肝癌模型。成瘤1周后,200μg/ml丝裂霉素C处理的2×107/ml Hepa1-6细胞感染LX/(IL-7)病毒制成肿瘤疫苗。对照组小鼠皮下接种丝裂霉素C处理的Hepa1-6细胞,每只接种1×106个细胞,实验组接种瘤苗,每只接种1×106个细胞,每周2次。测量肿瘤体积,通过流式检测小鼠脾脏细胞免疫反应。结果 LX/(IL-7)病毒具有非裂解性特征,可以高效地感染肝癌细胞并分泌IL-7。肝癌皮下模型的结果表明LX/(IL-7)修饰的Hepa1-6瘤苗具有较好的肿瘤免疫治疗效果,流式结果显示其可增强效应T细胞的比例,促进T细胞的免疫应答。结论携带IL-7的新城疫病毒修饰的自体肿瘤疫苗具有显著抗肝癌作用。
Objective To study the anti-hepatocarcinoma effect of Newcastle disease virus modified autologous tumor vaccine carrying IL-7. Methods Newcastle disease virus LX / (IL-7) was amplified from chicken embryos and Hepa1-6 cells were infected in vitro. The lytic characteristics of LX / (IL-7) were observed under microscope. C57BL / 6 mice were divided into two groups with 5 mice in each group. Mouse liver cancer model was established by subcutaneous inoculation of 5 × 106 Hepa1-6 cells. One week after tumorigenesis, 2 × 10 7 / ml Hepa 1-6 cells treated with 200 μg / ml mitomycin C were infected with LX / (IL-7) virus to prepare a tumor vaccine. The control mice were inoculated subcutaneously with mitomycin C-treated Hepa 1-6 cells, each of which was inoculated with 1 × 10 6 cells. The experimental group was inoculated with 1 × 10 6 cells per vaccination twice a week. Tumor volume was measured and the spleen cell immune response was detected by flow cytometry. Results The LX / (IL-7) virus has non-lytic characteristics and can effectively infect liver cancer cells and secrete IL-7. The results of the subcutaneous model of liver cancer showed that the Hepa1-6 tumor vaccine modified by LX / (IL-7) had better tumor immunotherapy effect, and the flow cytometry showed that it could enhance the proportion of effector T cells and promote the immune response of T cells. Conclusion Newcastle disease virus-modified autologous tumor vaccine carrying IL-7 has significant anti-hepatocellular carcinoma effect.