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目的 :了解异丙酚对大鼠脑一氧化氮 (NO) /环鸟苷酸 (cGMP)信号转导系统的影响。方法 :32只SD大鼠 ,随机分为对照组和异丙酚组 ,分别腹腔注射 (ip)生理盐水 10ml/kg或异丙酚 10 0mg/kg。用分光光度法测定脑组织一氧化氮合酶 (NOS)活性和NO产量 ,放射免疫法测定脑组织cGMP含量。结果 :与对照组比较 ,大鼠ip异丙酚 10 0mg/kg不但明显抑制小脑、海马和大脑皮层的NOS活性 ,而且显著减少上述脑区NO产量和cGMP含量 (P <0 0 5或P <0 0 1)。结论 :NO/cGMP信号转导系统可能在异丙酚的全麻分子学机理中发挥重要作用
Objective: To investigate the effect of propofol on signal transducers of nitric oxide (NO) / cGMP in rat brain. Methods: Thirty-two Sprague-Dawley rats were randomly divided into control group and propofol group. The animals were intraperitoneally injected with normal saline (10ml / kg) or propofol (100mg / kg) respectively. The activity of nitric oxide synthase (NOS) and NO production in brain tissue were measured by spectrophotometry. The content of cGMP in brain tissue was determined by radioimmunoassay. RESULTS: Compared with the control group, propofol ip at 100 mg / kg not only inhibited NOS activity in cerebellum, hippocampus and cerebral cortex, but also significantly reduced NO production and cGMP content (P <0.05 or P < 0 0 1). Conclusion: The NO / cGMP signal transduction system may play an important role in the molecular mechanism of general anesthesia with propofol